Rat FGF7 ELISA Kit | FGF7 elisa kit

Rat FGF7 (Fibroblast Growth Factor 7) ELISA Kit

Cat. Num. AAA2502003

• Gene Names: FGF7; KGF; HBGF-7
• Reactivity: Rat
• Synonyms: FGF7; Rat FGF7 (Fibroblast Growth Factor 7) ELISA Kit; FGF7 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat FGF7. No significant cross-reactivity or interference between Rat FGF7 and analogues was observed.
• Sequence Length: 194

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: Min: 9.375pg/mL; Max: 1000pg/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat FGF7 concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF7 elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to FGF7. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for FGF7 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain FGF7, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of FGF7. You can calculate the concentration of FGF7 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4503705
• NCBI GeneID: 2252
• NCBI Accession #: NP_002000.1
• NCBI GenBank Nucleotide #: NM_002009.3
• UniProt Accession #: P21781; Q6FGV5; Q96FG5; H0YNY5
• Molecular Weight: 11,520 Da
• NCBI Official Full Name: fibroblast growth factor 7
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: fibroblast growth factor 7; FGF-7; heparin-binding growth factor 7; keratinocyte growth factor
• UniProt Protein Name: Fibroblast growth factor 7
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF; FGF-7; HBGF-7
• UniProt Entry Name: FGF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of keratinocyte migration; epidermis development; phosphoinositide-mediated signaling; hair follicle morphogenesis; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; actin cytoskeleton reorganization; positive regulation of cell proliferation; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation