Mouse ELAV Like Protein 1 (ELAVL1) ELISA Kit | ELAVL1 elisa kit

Mouse ELAV Like Protein 1 (ELAVL1) ELISA Kit

Cat. Num. AAA457026

• Gene Names: Elavl1; HUR; Hua; W91709; 2410055N02Rik
• Reactivity: Mouse
• Synonyms: ELAV Like Protein 1 (ELAVL1); Mouse ELAV Like Protein 1 (ELAVL1) ELISA Kit; ELAV1; HUR; Hua; MelG; Hu Antigen R; Embryonic Lethal; Abnormal Vision Like 1; ELAVL1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ELAVL1. No significant cross-reactivity or interference between ELAVL1 and analogues was observed.
• Sequence Length: 326

• Samples: Tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.051ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ELAVL1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ELAVL1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ELAVL1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ELAVL1 in mouse tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ELAVL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ELAVL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ELAVL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ELAVL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31542602
• NCBI GeneID: 15568
• NCBI Accession #: NP_034615.2
• NCBI GenBank Nucleotide #: NM_010485.3
• UniProt Accession #: P70372; Q60745; Q78QY3
• Molecular Weight: 36,169 Da
• NCBI Official Full Name: ELAV-like protein 1
• NCBI Official Synonym Full Names: ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R)
• NCBI Official Symbol: Elavl1
• NCBI Official Synonym Symbols: HUR; Hua; W91709; 2410055N02Rik
• NCBI Protein Information: ELAV-like protein 1
• UniProt Protein Name: ELAV-like protein 1
• Protein Family: ELAV-like protein
• UniProt Gene Name: Elavl1
• UniProt Synonym Gene Names: Elra; Hua; HuR

Uniprot Description

RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability. Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (PubMed:24394384). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs. Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (PubMed:21402775).