Human Dihydrotestosterone (DHT) ELISA Kit | DHT elisa kit

Human Dihydrotestosterone (DHT) ELISA Kit

Cat. Num. AAA266535

• Reactivity: Human
• Synonyms: Dihydrotestosterone (DHT); Human Dihydrotestosterone (DHT) ELISA Kit; DHT elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 381

• Samples: Human serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 2000 pg/ml-31.2 pg/ml
• Sensitivity: Up to 12 pg/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DHT elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Human DHT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 1903379
• Molecular Weight: 43,009 Da
• NCBI Official Full Name: differentially repressed by testosterone and dihydrotestosterone
• UniProt Protein Name: Protein NDRG1
• Protein Family: D-hydantoinase/dihydropyrimidinase
• UniProt Gene Name: Ndrg1
• UniProt Synonym Gene Names: Ndr1; Ndrl; Tdd5; Protein Ndr1
• UniProt Entry Name: NDRG1_MOUSE

Uniprot Description

NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).

Protein type: Vesicle; Cell development/differentiation

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; cytoskeleton; membrane; perinuclear region of cytoplasm; recycling endosome membrane; cytoplasm; plasma membrane; nucleus; myelin sheath

Molecular Function: gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding

Biological Process: myelin maintenance in the peripheral nervous system; mast cell activation