Rat Defensin Alpha 1 (DEFa1) ELISA Kit | DEFa1 elisa kit

Rat Defensin Alpha 1 (DEFa1) ELISA Kit

Cat. Num. AAA2707549

• Gene Names: DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• Reactivity: Rat
• Synonyms: Defensin Alpha 1 (DEFa1); Rat Defensin Alpha 1 (DEFa1) ELISA Kit; DEF1; DEFA2; HNP-1; HP-1; MRS; DEF3; HNP-3; HNP3; HP-3; Defensin;Alpha 1; Myeloid-Related Sequence; DEFa1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of DEFa1. No significant cross-reactivity or interference between DEFa1 and analogues was observed.
• Sequence Length: 94

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.117ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DEFa1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DEFa1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFa1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DEFa1 in rat serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DEFa1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DEFa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEFa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DEFa1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 50057842
• NCBI GeneID: 1667
• NCBI Official Full Name: defensin, alpha 1, myeloid-related sequence
• NCBI Official Synonym Full Names: defensin alpha 1
• NCBI Official Symbol: DEFA1
• NCBI Official Synonym Symbols: HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• NCBI Protein Information: neutrophil defensin 1
• Protein Family: Alpha-defensin

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, Oct 2014]