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Typical Testing Data/Standard Curve (for reference only)

Human Defensin Alpha 1, Neutrophil (DEFa1) RTU ELISA Kit | DEFa1 rtu elisa kit

Human Defensin Alpha 1, Neutrophil (DEFa1) ELISA Kit

Gene Names
DEFA1B; HP1; HP-1; HNP-1
Reactivity
Human
Synonyms
Defensin Alpha 1; Neutrophil (DEFa1); Human Defensin Alpha 1; Neutrophil (DEFa1) ELISA Kit; DEF1; DEFA2; HNP-1; HP-1; MRS; DEF3; HNP-3; HNP3; HP-3; Defensin; Alpha 1; Myeloid-Related Sequence; DEFa1 rtu elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
94
Assay Type
Sandwich
Samples
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
Detection Range
0.312-20ng/mL
Sensitivity
0.146ng/mL
RTU ELISA Kit's Advantage
The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
Preparation and Storage
For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DEFa1 rtu elisa kit
Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEFa1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DEFa1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEFa1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DEFa1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,201 Da
NCBI Official Full Name
neutrophil defensin 1 isoform 2 preproprotein
NCBI Official Synonym Full Names
defensin alpha 1B
NCBI Official Symbol
DEFA1B
NCBI Official Synonym Symbols
HP1; HP-1; HNP-1
NCBI Protein Information
neutrophil defensin 1
UniProt Protein Name
Neutrophil defensin 1
Protein Family
UniProt Gene Name
DEFA1
UniProt Synonym Gene Names
DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2014]

Uniprot Description

Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram-negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane.

Research Articles on DEFa1

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Product Notes

The Human DEFa1 defa1 (Catalog #AAA458814) is a RTU ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA458814 RTU ELISA Kit recognizes Human DEFa1. It is sometimes possible for the material contained within the vial of "Defensin Alpha 1, Neutrophil (DEFa1), RTU ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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