Rat Cyclin-dependent kinase 3 (CDK3) ELISA Kit | CDK3 elisa kit

Rat Cyclin-dependent kinase 3 (CDK3) ELISA Kit

Cat. Num. AAA7205613

• Reactivity: Rat
• Synonyms: Cyclin-dependent kinase 3 (CDK3); Rat Cyclin-dependent kinase 3 (CDK3) ELISA Kit; CDK3 elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for CDK3 elisa kit

Intended Uses: This CDK3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat CDK3. This ELISA kit for research use only!

Principle of the Assay||CDK3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDK3 antibody and an CDK3-HRP conjugate. The assay sample and buffer are incubated together with CDK3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDK3 concentration since CDK3 from samples and CDK3-HRP conjugate compete for the anti-CDK3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDK3 from the sample, fewer sites are left to bind CDK3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDK3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CDK3 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 4557439
• NCBI GeneID: 1018
• NCBI Accession #: NP_001249.1
• NCBI GenBank Nucleotide #: NM_001258.2
• Molecular Weight: 35,046 Da
• NCBI Official Full Name: cyclin-dependent kinase 3
• NCBI Official Synonym Full Names: cyclin-dependent kinase 3
• NCBI Official Symbol: CDK3
• NCBI Protein Information: cyclin-dependent kinase 3; cell division protein kinase 3
• UniProt Protein Name: Cyclin-dependent kinase 3
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK3
• UniProt Synonym Gene Names: CDKN3
• UniProt Entry Name: CDK3_HUMAN

NCBI Description

This gene encodes a member of the cyclin-dependent protein kinase family. The protein promotes entry into S phase, in part by activating members of the E2F family of transcription factors. The protein also associates with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK3: a protein kinase of the CDK family. May be involved in cell cycle control. Can phosphorylate histone H1.

Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.22; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 17q25.1

Molecular Function: cyclin-dependent protein kinase activity; ATP binding

Biological Process: mitosis; cell proliferation; G0 to G1 transition; regulation of cell cycle; cell division; protein amino acid phosphorylation; G1/S transition of mitotic cell cycle