Typical Testing Data/Standard Curve
Canine B1 bradykinin receptor (BDKRB1) ELISA Kit | BDKRB1 elisa kit
Canine B1 bradykinin receptor (BDKRB1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||BDKRB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-BDKRB1 antibody and an BDKRB1-HRP conjugate. The assay sample and buffer are incubated together with BDKRB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the BDKRB1 concentration since BDKRB1 from samples and BDKRB1-HRP conjugate compete for the anti-BDKRB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by BDKRB1 from the sample, fewer sites are left to bind BDKRB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The BDKRB1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Bradykinin, a 9 aa peptide, is generated in pathophysiologic conditions such as inflammation, trauma, burns, shock, and allergy. Two types of G-protein coupled receptors have been found which bind bradykinin and mediate responses to these pathophysiologic conditions. The protein encoded by this gene is one of these receptors and is synthesized de novo following tissue injury. Receptor binding leads to an increase in the cytosolic calcium ion concentration, ultimately resulting in chronic and acute inflammatory responses. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
BKR1: This is a receptor for bradykinin. Could be a factor in chronic pain and inflammation. Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB1 sub-subfamily.
Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 14q32.1-q32.2
Cellular Component: neuron projection; integral to plasma membrane; endoplasmic reticulum; plasma membrane
Molecular Function: bradykinin receptor activity; protein binding; peptide binding
Biological Process: elevation of cytosolic calcium ion concentration; cell migration; negative regulation of protein amino acid phosphorylation; response to mechanical stimulus; positive regulation of leukocyte migration; protein kinase C activation; response to lipopolysaccharide; negative regulation of blood pressure; negative regulation of cell growth; positive regulation of release of sequestered calcium ion into cytosol; inflammatory response; sensory perception of pain
Research Articles on BDKRB1
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Product Notes
The Canine BDKRB1 bdkrb1 (Catalog #AAA7233784) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7233784 ELISA Kit recognizes Canine BDKRB1. It is sometimes possible for the material contained within the vial of "B1 bradykinin receptor (BDKRB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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