Rat CTSB ELISA Kit | CTSB elisa kit

Rat CTSB (Cathepsin B) ELISA Kit

Cat. Num. AAA2515732

• Gene Names: CTSB; APPS; CPSB
• Reactivity: Rat
• Synonyms: CTSB; Rat CTSB (Cathepsin B) ELISA Kit; CTSB elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinant Rat CTSB. No significant cross-reactivity or interference between Rat CTSB and analogues was observed.
• Sequence Length: 339

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: Min: 0.094ng/mL; Max: 10ng/mL
• Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Rat CTSB concentrations in serum, plasma and other biological fluids.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSB elisa kit

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to CTSB. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for CTSB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain CTSB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of CTSB. You can calculate the concentration of CTSB in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22538437
• NCBI GeneID: 1508
• NCBI Accession #: NP_680093.1
• NCBI GenBank Nucleotide #: NM_147783.2
• Molecular Weight: 37,822 Da
• NCBI Official Full Name: cathepsin B preproprotein
• NCBI Official Synonym Full Names: cathepsin B
• NCBI Official Symbol: CTSB
• NCBI Official Synonym Symbols: APPS; CPSB
• NCBI Protein Information: cathepsin B; APP secretase; amyloid precursor protein secretase; cathepsin B1; cysteine protease
• UniProt Protein Name: Cathepsin B
• Protein Family: Cathepsin
• UniProt Gene Name: CTSB
• UniProt Synonym Gene Names: CPSB; APPS
• UniProt Entry Name: CATB_HUMAN

NCBI Description

The protein encoded by this gene is a lysosomal cysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is also known as amyloid precursor protein secretase and is involved in the proteolytic processing of amyloid precursor protein (APP). Incomplete proteolytic processing of APP has been suggested to be a causative factor in Alzheimer disease, the most common cause of dementia. Overexpression of the encoded protein, which is a member of the peptidase C1 family, has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSB: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Dimer of a heavy chain and a light chain cross-linked by a disulfide bond. Interacts with SRPX2. Belongs to the peptidase C1 family.

Protein type: Protease; Motility/polarity/chemotaxis; EC 3.4.22.1; Autophagy

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: extracellular space; intracellular membrane-bound organelle; mitochondrion; perinuclear region of cytoplasm; lysosome; nucleolus; extracellular region; melanosome; intracellular

Molecular Function: peptidase activity; collagen binding; proteoglycan binding; protein binding; cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; proteolysis involved in cellular protein catabolic process; epithelial cell differentiation; regulation of catalytic activity; toll-like receptor signaling pathway; innate immune response; decidualization; proteolysis