Typical Testing Data/Standard Curve (for reference only)
Rat CREB Binding Protein (CREBBP) ELISA Kit | CREBBP elisa kit
Rat CREB Binding Protein (CREBBP) ELISA Kit
Gene Names
Crebbp; CBP; RTS; RSTS
Reactivity
Rat
Synonyms
CREB Binding Protein (CREBBP); Rat CREB Binding Protein (CREBBP) ELISA Kit; CBP; RSTS; RTS; KAT3A; Rubinstein-Taybi Syndrome; CREBBP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CREBBP. No significant cross-reactivity or interference between CREBBP and analogues was observed.
Sequence Length
2442
Samples
Rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.114ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CREBBP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CREBBP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CREBBP elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CREBBP in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CREBBP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CREBBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CREBBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CREBBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CREBBP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CREBBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CREBBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CREBBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for CREBBP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
265,424 Da
NCBI Official Full Name
CREB-binding protein
NCBI Official Synonym Full Names
CREB binding protein
NCBI Official Symbol
Crebbp
NCBI Official Synonym Symbols
CBP; RTS; RSTS
NCBI Protein Information
CREB-binding protein
UniProt Protein Name
CREB-binding protein
Protein Family
UniProt Gene Name
Crebbp
UniProt Synonym Gene Names
Cbp
NCBI Description
a transcriptional co-activator; has an important role in hormone-dependent female sexual behavior and long term memory [RGD, Feb 2006]
Uniprot Description
Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER).
Research Articles on CREBBP
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Product Notes
The Rat CREBBP crebbp (Catalog #AAA2098054) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2098054 ELISA Kit recognizes Rat CREBBP. It is sometimes possible for the material contained within the vial of "CREB Binding Protein (CREBBP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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