Mouse Methyl CpG Binding Protein 2 (MECP2) RTU ELISA Kit | MECP2 rtu elisa kit

Mouse Methyl CpG Binding Protein 2 (MECP2) ELISA Kit

Cat. Num. AAA4500975

• Gene Names: Mecp2; Mbd5; WBP10; 1500041B07Rik; D630021H01Rik
• Reactivity: Mouse
• Synonyms: Methyl CpG Binding Protein 2 (MECP2); Mouse Methyl CpG Binding Protein 2 (MECP2) ELISA Kit; RTS; AUTSX3; MRX16; MRX79; PPMX; RTT; Rett Syndrome; Mental Retardation; X-linked 16; MECP2 rtu elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of MECP2. No significant cross-reactivity or interference between MECP2 and analogues was observed.
• Sequence Length: 501

• Samples: Mouse tissue homogenates, cell lysates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.102ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MECP2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MECP2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MECP2 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of MECP2 in mouse tissue homogenates, cell lysates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MECP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MECP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MECP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MECP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 126517469
• NCBI GeneID: 17257
• NCBI Accession #: NP_001075448.1
• NCBI GenBank Nucleotide #: NM_001081979.2
• UniProt Accession #: Q9Z2D6; Q3TYG1; B1AUZ2; B1AUZ3
• Molecular Weight: 53,576 Da
• NCBI Official Full Name: methyl-CpG-binding protein 2 isoform 1
• NCBI Official Synonym Full Names: methyl CpG binding protein 2
• NCBI Official Symbol: Mecp2
• NCBI Official Synonym Symbols: Mbd5; WBP10; 1500041B07Rik; D630021H01Rik
• NCBI Protein Information: methyl-CpG-binding protein 2
• UniProt Protein Name: Methyl-CpG-binding protein 2
• Protein Family: Methyl-CpG-binding protein
• UniProt Gene Name: Mecp2
• UniProt Synonym Gene Names: MeCp-2 protein; MeCp2

Uniprot Description

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).