Rat Cannabinoid Receptor 1, Brain (CNR1) ELISA Kit | CNR1 elisa kit

Rat Cannabinoid Receptor 1, Brain (CNR1) ELISA Kit

Cat. Num. AAA2023659

• Gene Names: Cnr1; SKR6R
• Reactivity: Rat
• Synonyms: Cannabinoid Receptor 1; Brain (CNR1); Rat Cannabinoid Receptor 1; Brain (CNR1) ELISA Kit; CANN6; CB-R; CB1; CB1A; CB1K5; CNR; CNR1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cannabinoid Receptor 1, Brain (CNR1).; No significant cross-reactivity or interference between Cannabinoid Receptor 1, Brain (CNR1) and analogues was observed.
• Sequence Length: 473

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.114ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cannabinoid Receptor 1, Brain (CNR1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cannabinoid Receptor 1, Brain (CNR1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNR1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cannabinoid Receptor 1, Brain (CNR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cannabinoid Receptor 1, Brain (CNR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cannabinoid Receptor 1, Brain (CNR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cannabinoid Receptor 1, Brain (CNR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CNR1 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 6978673
• NCBI GeneID: 25248
• NCBI Accession #: NP_036916.1
• NCBI GenBank Nucleotide #: NM_012784.4
• UniProt Accession #: P20272
• Molecular Weight: 52,845 Da
• NCBI Official Full Name: cannabinoid receptor 1
• NCBI Official Synonym Full Names: cannabinoid receptor 1 (brain)
• NCBI Official Symbol: Cnr1
• NCBI Official Synonym Symbols: SKR6R
• NCBI Protein Information: cannabinoid receptor 1
• UniProt Protein Name: Cannabinoid receptor 1
• Protein Family: Cannabinoid receptor
• UniProt Gene Name: Cnr1
• UniProt Synonym Gene Names: Skr6; CB-R; CB1
• UniProt Entry Name: CNR1_RAT

NCBI Description

G-protein coupled receptor for psychoactive cannabinoids; may mediate inhibition of NO production and participate in retrograde signaling by endocannabinoids [RGD, Feb 2006]

Uniprot Description

CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Cellular Component: growth cone; intracellular membrane-bound organelle; axon; integral to membrane; plasma membrane

Molecular Function: protein binding; cannabinoid receptor activity; drug binding

Biological Process: response to nicotine; negative regulation of fatty acid beta-oxidation; regulation of synaptic transmission, glutamatergic; regulation of lipid metabolic process; maternal process involved in pregnancy; positive regulation of apoptosis; response to morphine; positive regulation of acute inflammatory response to antigenic stimulus; response to lipopolysaccharide; sensory perception of pain; glucose homeostasis; learning and/or memory; negative regulation of blood pressure; negative regulation of dopamine secretion; response to nutrient; aging; negative regulation of mast cell activation; negative regulation of nitric-oxide synthase activity; axonal fasciculation; negative regulation of action potential; response to cocaine; memory; regulation of synaptic transmission, GABAergic; G-protein signaling, coupled to cAMP nucleotide second messenger; G-protein coupled receptor protein signaling pathway; response to ethanol; positive regulation of fever; spermatogenesis; regulation of insulin secretion; negative regulation of ion transport; positive regulation of blood pressure