Rat Corticotropin Releasing Hormone Binding Protein (CRHBP) ELISA Kit | CRHBP elisa kit

Rat Corticotropin Releasing Hormone Binding Protein (CRHBP) ELISA Kit

Cat. Num. AAA450870

• Gene Names: Crhbp; Crfbp
• Reactivity: Rat
• Synonyms: Corticotropin Releasing Hormone Binding Protein (CRHBP); Rat Corticotropin Releasing Hormone Binding Protein (CRHBP) ELISA Kit; CRF-BP; CRFBP; Corticotropin-Releasing Factor-Binding Protein; CRHBP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRHBP. No significant cross-reactivity or interference between CRHBP and analogues was observed.
• Sequence Length: 322

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 15.625-1000pg/mL
• Sensitivity: 5.9pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRHBP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRHBP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Standard Curve/Testing Data

Related Product Information for CRHBP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRHBP in rat serum, plasma other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRHBP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CRHBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRHBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRHBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21070952
• NCBI GeneID: 29625
• NCBI Accession #: NP_631922.1
• NCBI GenBank Nucleotide #: NM_139183.2
• UniProt Accession #: O35761; P24388
• Molecular Weight: 36,151 Da
• NCBI Official Full Name: corticotropin-releasing factor-binding protein
• NCBI Official Synonym Full Names: corticotropin releasing hormone binding protein
• NCBI Official Symbol: Crhbp
• NCBI Official Synonym Symbols: Crfbp
• NCBI Protein Information: corticotropin-releasing factor-binding protein
• UniProt Protein Name: Corticotropin-releasing factor-binding protein
• Protein Family: Corticotropin-releasing factor-binding protein
• UniProt Gene Name: Crhbp
• UniProt Synonym Gene Names: CRF-BP; CRF-binding protein

NCBI Description

binds corticotropin releasing hormone and modulates CRH action; may act as a reservoir of endogenous CRH in the brain [RGD, Feb 2006]

Uniprot Description

Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy.