Typical Testing Data/Standard Curve (for reference only)
Rat Collagen Type III, Col III ELISA Kit | Col III elisa kit
Rat Collagen Type III, Col III ELISA Kit
Reactivity
Rat
Synonyms
Collagen Type III; Col III; Rat Collagen Type III; Col III ELISA Kit; Col III ELISA KIT; Col III elisa kit
Reactivity
Rat
Samples
Serum, plasma, tissue homogenates
Detection Range
0.156 ng/ml-10 ng/ml
Sensitivity
0.039 ng/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Col III elisa kit
Introduction: Type III collagen has been suggested to play a major role in development and wound healing, and contributestothe viscoelastic properties of mostconnective tissues. Type III Collagen is found co-localized with Type I Collagen in tissues, blood vessels and skin. Type III Collagen is a homotrimeric procollagen comprised of three identical pro alpha1 (III) chains. Collagen III is important for the development of skin and the cardiovascular system and for maintaining normal physiological functions in adult life. Collagen III has been used for studies of Collagen I fibrillogenesis in normal cardiovascular development. Many of the same properties and functions of animal derived collagens can be duplicated using recombinant human collagens
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Col III. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Col III and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Col III, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Col III in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Col III. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Col III and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Col III, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Col III in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Similar Products
Product Notes
The Rat Col III (Catalog #AAA703199) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703199 ELISA Kit recognizes Rat Col III. It is sometimes possible for the material contained within the vial of "Collagen Type III, Col III, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
