Typical Testing Data/Standard Curve (for reference only)
Mouse Fibrinogen, Fbg ELISA Kit | Fbg elisa kit
Mouse Fibrinogen, Fbg ELISA Kit
Reactivity
Mouse
Synonyms
Fibrinogen; Fbg; Mouse Fibrinogen; Fbg ELISA Kit; Fbg elisa kit
Reactivity
Mouse
Sequence Length
444
Samples
Serum, plasma and tissue homogenates
Detection Range
0.156 ug/ml-10 ug/ml
Sensitivity
0.039 ug/ml
Detection Wavelength
450 nm
Sample Volume
50-100ul
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Fbg elisa kit
Introduction: Fibrinogen is a soluble plasma glycoprotein, synthesised by the liver, that is converted by thrombin into fibrin during blood coagulation. Processes in the coagulation cascade activate the zymogen prothrombin to the serine protease thrombin, which is responsible for converting fibrinogen into fibrin. Fibrin is then cross linked by factor XIII to form a clot. FXIIIa stabilizes fibrin further by incorporation of the fibrinolysis inhibitors alpha-2-antiplasmin and TAFI, and binding to several adhesive proteins of various cells. Both the activation of Factor XIII by thrombin and plasminogen activator (t-PA) are catalyzed by fibrin. Fibrin specifically binds the activated coagulation factors factor Xa and thrombin and entraps them in the network of fibers, thus functioning as a temporary inhibitor of these enzymes which stay active and can be released during fibrinolysis. Recent research has shown that fibrin plays a key role in the inflammatory response and development of rheumatoid arthritis.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FBG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to FBG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FBG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain FBG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of FBG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
50,244 Da
NCBI Official Full Name
fibrinogen
NCBI Official Synonym Full Names
fibrinogen gamma chain
NCBI Official Symbol
FGG
NCBI Protein Information
fibrinogen gamma-B chain; gamma'; fibrinogen, gamma polypeptide
UniProt Protein Name
Fibrinogen gamma-B chain
UniProt Gene Name
FGG
UniProt Synonym Gene Names
Gamma'
UniProt Entry Name
FIBG_BOVIN
Uniprot Description
Function: Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.
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Product Notes
The Mouse Fbg fgg (Catalog #AAA704989) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA704989 ELISA Kit recognizes Mouse Fbg. It is sometimes possible for the material contained within the vial of "Fibrinogen, Fbg, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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