Rabbit GLP1 Polyclonal Antibody | anti-GLP1 antibody

Anti-GLP1 Antibody

Cat. Num. AAA177908

• Gene Names: GCG; GLP1; GLP2; GRPP
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Purity: Immunogen Affinity Purified
• Synonyms: GLP1; Polyclonal Antibody; Anti-GLP1 Antibody; Glucagon; GCG; glicentin-related polypeptide; GLP-1; GLP-1(7-36); GLP-1(7-37); GLP-2; included; GLP2; GLUC_HUMAN; Glucagon like peptide 1; glucagon-like peptide 1; Glucagon-like peptide 1; Glucagon-like peptide 2; GRPP; OXM; OXY; preproglucagon; glucagon; anti-GLP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: No cross reactivity with other proteins
• Purity/Purification: Immunogen Affinity Purified
• Form/Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.
• Sequence Length: 180

• Applicable Applications for anti-GLP1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (IHC) Paraffin:; Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat; ; Testes Species: In-house tested species with positive results; By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.; ; Other applications have not been tested.; ptimal dilutions should be determined by end users.
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human GLP1 (53-81aa HSQGTFTSDYSKYLDSRRAQDFVQWLMNT), identical to the related mouse and rat sequences.
• Reconstitution: 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Relevent Detection Systems: Antibody can be supported by MBS176451 in IHC(P).
• Preparation and Storage: Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti- GLP1 Picoband antibody, MBS177908, IHC(P)IHC(P): Mouse Pancreas Tissue )

Immunohistochemistry (IHC)

(Anti- GLP1 Picoband antibody, MBS177908, IHC(P)IHC(P): Rat Pancreas Tissue )

Immunohistochemistry (IHC)

(Anti- GLP1 Picoband antibody, MBS177908, IHC(P)IHC(P): Human Pancreatic Cancer Tissue )

Related Product Information for anti-GLP1 antibody

Description: Rabbit IgG polyclonal antibody for Glucagon(GCG) detection. Tested with IHC-P in Human;Mouse;Rat.

Background: GCG is also known as GLP1, or Glucagon. Glucagon is a 29-amino acid pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. It is mapped to 2q36-2q37. GLP1, also known as 7-37 for the codons of the preproglucagon molecule which encode it, renders pancreatic beta-cells 'glucose-competent' and may be useful in the treatment of noninsulin-dependent diabetes mellitus. Also, GLP1 is a potent insulin secretagogue. It plays a major role in the enteroinsular axis, accounting, for example, for the finding that plasma insulin levels accompanying oral intake of glucose are greater than those observed when glucose is given intravenously.

References

1. Arora S, Galich P (Mar 2009). "Myth: glucagon is an effective first-line therapy for esophageal foreign body impaction". Cjem 11 (2): 169-71. 2. Holz, G. G., IV, Kuhtreiber, W. M., Habener, J. F. Pancreatic beta-cells are rendered glucose-competent by the insulinotropic hormone glucagon-like peptide-1 (7-37). Nature 361: 362-365, 1993.  3. Liljenquist JE, Bomboy JD, Lewis SB, Sinclair-Smith BC, Felts PW, Lacy WW, Crofford OB, Liddle GW (Jan 1974). "Effects of glucagon on lipolysis and ketogenesis in normal and diabetic men" (PDF). The Journal of Clinical Investigation 53 (1): 190-7.

NCBI and Uniprot Product Information

• NCBI GI #: 4503945
• NCBI GeneID: 2641
• NCBI Accession #: NP_002045.1
• NCBI GenBank Nucleotide #: NM_002054.4
• UniProt Accession #: P01275; Q53TP6; A6NN65
• Molecular Weight: 20,909 Da
• NCBI Official Full Name: glucagon preproprotein
• NCBI Official Synonym Full Names: glucagon
• NCBI Official Symbol: GCG
• NCBI Official Synonym Symbols: GLP1; GLP2; GRPP
• NCBI Protein Information: glucagon
• UniProt Protein Name: Glucagon
• Protein Family: Germin-like protein
• UniProt Gene Name: GCG
• UniProt Synonym Gene Names: GRPP; OXM; OXY; GLP-1; GLP-1(7-37); GLP-1(7-36); GLP-2
• UniProt Entry Name: GLUC_HUMAN

NCBI Description

The protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon. [provided by RefSeq, Jul 2008]

Uniprot Description

GCG: Glucagon plays a key role in glucose metabolism and homeostasis. Regulates blood glucose by increasing gluconeogenesis and decreasing glycolysis. A counterregulatory hormone of insulin, raises plasma glucose levels in response to insulin-induced hypoglycemia. Plays an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. GLP-1 and GLP-2 are induced in response to nutrient ingestion. Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain. Belongs to the glucagon family.

Protein type: Secreted, signal peptide; Secreted; Hormone

Chromosomal Location of Human Ortholog: 2q36-q37

Cellular Component: endoplasmic reticulum lumen; extracellular region; extracellular space; plasma membrane

Molecular Function: glucagon receptor binding; hormone activity; identical protein binding; protein binding; receptor binding

Biological Process: cell proliferation; feeding behavior; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; negative regulation of apoptosis; negative regulation of appetite; positive regulation of cAMP biosynthetic process; positive regulation of histone H3-K4 methylation; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein binding; positive regulation of protein kinase activity; regulation of insulin secretion; response to starvation; signal transduction