Rat Cluster Of Differentiation 30 (CD30) ELISA Kit | CD30 elisa kit

Rat Cluster Of Differentiation 30 (CD30) ELISA Kit

Cat. Num. AAA9711671

• Gene Names: Tnfrsf8; Cd30
• Reactivity: Rat
• Synonyms: Cluster Of Differentiation 30 (CD30); Rat Cluster Of Differentiation 30 (CD30) ELISA Kit; TNFRSF8; CD30; D1S166E; Ki-1; CD30 antigen; CD30L receptor; Ki-1 antigen; cytokine receptor CD30; lymphocyte activation antigen CD30; CD30 elisa kit

• Reactivity: Rat
• Specificity: Rat Cluster of differentiation 30 (CD30) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFRSF8. No significant cross-reactivity or interference between Rat TNFRSF8 and analogues was observed.
• Sequence Length: 493

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Cluster of differentiation 30 (CD30) Standard Curve.)

Related Product Information for CD30 elisa kit

Description: This Rat Cluster of differentiation 30 (CD30) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF8 in samples. An antibody specific for TNFRSF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFRSF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: This protein is a member of the TNF-receptor superfamily. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 9507193
• NCBI GeneID: 25069
• NCBI Accession #: NP_062008.1
• NCBI GenBank Nucleotide #: NP_062008.1
• UniProt Accession #: P97525
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 8
• NCBI Official Synonym Full Names: TNF receptor superfamily member 8
• NCBI Official Symbol: Tnfrsf8
• NCBI Official Synonym Symbols: Cd30
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 8
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 8
• UniProt Gene Name: Tnfrsf8
• UniProt Synonym Gene Names: Cd30

NCBI Description

Tnfsf8/Cd30l receptor [RGD, Feb 2006]

Uniprot Description

Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B ().