Rabbit anti-Human FZD9 Polyclonal Antibody | anti-FZD9 antibody

FZD9, NT (FZD9, FZD3, Frizzled-9, FzE6, CD349) (Azide free) (HRP)

Cat. Num. AAA6300613

• Gene Names: FZD9; FZD3; CD349
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: FZD9; Polyclonal Antibody; NT (FZD9; FZD3; Frizzled-9; FzE6; CD349) (Azide free) (HRP); CD349; anti-FZD9 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-FZD9 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: FZD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-35 amino acids from the N-terminal region of human FZD9.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(FZD9 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the FZD9 antibody detected the FZD9 protein (arrow).)

Related Product Information for anti-FZD9 antibody

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.

Product Categories/Family for anti-FZD9 antibody

Antibodies; Wnt Proteins

References

Trubiani, O., et al. J. Cell. Physiol. 225(1):123-131(2010). Saus, E., et al. J Psychiatr Res (2010). In press: Fujimoto, T., et al. Int. J. Oncol. 35(4):861-866(2009). Kim, J.G., et al. J. Korean Med. Sci. 24(3):443-447(2009). Wang, H.X., et al. Mol. Hum. Reprod. 15(1):11-17(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503835
• NCBI GeneID: 8326
• NCBI Accession #: NP_003499.1
• NCBI GenBank Nucleotide #: NM_003508.2
• UniProt Accession #: O00144
• Molecular Weight: 64,466 Da
• NCBI Official Full Name: frizzled-9
• NCBI Official Synonym Full Names: frizzled family receptor 9
• NCBI Official Symbol: FZD9
• NCBI Official Synonym Symbols: FZD3; CD349
• NCBI Protein Information: frizzled-9; fz-9; fzE6; hFz9; frizzled homolog 9; frizzled 9, seven transmembrane spanning receptor
• UniProt Protein Name: Frizzled-9
• Protein Family: Frizzled
• UniProt Gene Name: FZD9
• UniProt Synonym Gene Names: FZD3; Fz-9; hFz9
• UniProt Entry Name: FZD9_HUMAN

NCBI Description

Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney. [provided by RefSeq, Jul 2008]

Uniprot Description

FZD9: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Belongs to the G-protein coupled receptor Fz/Smo family.

Protein type: Membrane protein, integral; GPCR, Fz/Smo family; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: filopodium membrane; cell surface; perinuclear region of cytoplasm; cytoplasm; integral to membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein homodimerization activity; protein heterodimerization activity

Biological Process: nervous system development; G-protein coupled receptor protein signaling pathway; learning and/or memory; B cell differentiation; neuroblast proliferation; Wnt receptor signaling pathway through beta-catenin