Rat Cholecystokinin A Receptor (CCKAR) ELISA Kit | CCKAR elisa kit

Rat Cholecystokinin A Receptor (CCKAR) ELISA Kit

Cat. Num. AAA453415

• Gene Names: Cckar; Cck-ar
• Reactivity: Rat
• Synonyms: Cholecystokinin A Receptor (CCKAR); Rat Cholecystokinin A Receptor (CCKAR) ELISA Kit; CCK-A; CCK1; CCKRA; CCK1R; Cholecystokinin Type 1 Receptor; CCKAR elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CCKAR. No significant cross-reactivity or interference between CCKAR and analogues was observed.
• Sequence Length: 444

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.079ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CCKAR were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CCKAR were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCKAR elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CCKAR in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CCKAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CCKAR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CCKAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CCKAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6978615
• NCBI GeneID: 24889
• NCBI Accession #: NP_036820.1
• NCBI GenBank Nucleotide #: NM_012688.3
• UniProt Accession #: P30551
• Molecular Weight: 49,657 Da
• NCBI Official Full Name: cholecystokinin receptor type A
• NCBI Official Synonym Full Names: cholecystokinin A receptor
• NCBI Official Symbol: Cckar
• NCBI Official Synonym Symbols: Cck-ar
• NCBI Protein Information: cholecystokinin receptor type A
• UniProt Protein Name: Cholecystokinin receptor type A
• Protein Family: Cholecystokinin receptor
• UniProt Gene Name: Cckar
• UniProt Synonym Gene Names: CCK-A receptor; CCK-AR; CCK1-R

NCBI Description

G-protein coupled receptor activated by cholecystokinin; may be involved in food intake and insulin regulation [RGD, Feb 2006]

Uniprot Description

Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.