Rat CD36/SR-B3 ELISA Kit | CD36 elisa kit

Rat CD36/SR-B3 PicoKine ELISA Kit

Cat. Num. AAA177246

• Gene Names: Cd36; Fat
• Reactivity: Rat
• Synonyms: CD36/SR-B3; Rat CD36/SR-B3 PicoKine ELISA Kit; Platelet glycoprotein 4; CD36 molecule (thrombospondin receptor); CD36 antigen; CD36 antigen (collagen type I receptor thrombospondin receptor); CD36 molecule; CHDS7; Cluster determinant 36; FAT; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; Leukocyte differentiation antigen; CD36; PAS 4 protein; PAS IV; PASIV; Platelet collagen receptor; Platelet glycoprotein IV; SCARB3; Scavenger receptor class B member 3; Thrombospondin receptor; CD36 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat CD36
• Sequence Length: 472

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 218pg/ml-14,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Rat CD36/SR-B3 PicoKine ELISA Kit standard curve )

Related Product Information for CD36 elisa kit

Principle of the assay: rat CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD36 has been precoated onto 96-well plates. Standards(CHO, G30-K439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CD36 amount of sample captured in plate.
Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

NCBI and Uniprot Product Information

• NCBI GI #: 146345388
• NCBI GeneID: 29184
• UniProt Accession #: Q07969; Q925W0
• Molecular Weight: 52,731 Da
• NCBI Official Full Name: Platelet glycoprotein 4
• NCBI Official Synonym Full Names: CD36 molecule (thrombospondin receptor)
• NCBI Official Symbol: Cd36
• NCBI Official Synonym Symbols: Fat
• NCBI Protein Information: platelet glycoprotein 4; GPIV; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV; adipocyte membrane protein; fatty acid transport protein; collagen type I receptor thrombospondin receptor; CD36 antigen (collagen type I receptor, thrombospondin receptor)
• UniProt Protein Name: Platelet glycoprotein 4
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: Cd36
• UniProt Synonym Gene Names: Fat; GPIIIB; GPIV
• UniProt Entry Name: CD36_RAT

NCBI Description

fatty acid translocase; involved in long-chain fatty acid (LCFA) transport; important in fatty acid metabolism and insulin function [RGD, Feb 2006]

Uniprot Description

CD36: Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is internalized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion

Protein type: Cell adhesion; Membrane protein, multi-pass; Membrane protein, integral

Cellular Component: Golgi apparatus; extracellular space; protein complex; cell surface; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; caveola; lipid raft; membrane; apical part of cell; plasma membrane; intracellular; sarcolemma; external side of plasma membrane

Molecular Function: low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding; low-density lipoprotein binding; lipid binding; high-density lipoprotein binding

Biological Process: positive regulation of blood coagulation; cGMP-mediated signaling; positive regulation of interleukin-12 production; phagocytosis, recognition; pattern recognition receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; signal transduction; negative regulation of transcription factor import into nucleus; fatty acid metabolic process; response to estradiol stimulus; low density lipoprotein mediated signaling; sequestering of lipid; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; cell adhesion; positive regulation of cell-matrix adhesion; response to nutrient; long-chain fatty acid transport; response to drug; receptor-mediated endocytosis; cholesterol transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of systemic arterial blood pressure; positive regulation of interleukin-6 production; long-chain fatty acid metabolic process; positive regulation of tumor necrosis factor production; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; triacylglycerol metabolic process; cellular response to insulin stimulus; gut development; response to mechanical stimulus; lipoprotein transport; fatty acid oxidation; response to activity; positive regulation of phagocytosis, engulfment; nitric oxide mediated signal transduction; plasma membrane long-chain fatty acid transport; apoptotic cell clearance; fatty acid transport; positive regulation of cytokine secretion