Mouse CD36/SR-B3 ELISA Kit | CD36 elisa kit

Mouse CD36/SR-B3 PicoKine ELISA Kit

Cat. Num. AAA177155

• Gene Names: Cd36; FAT; GPIV; Scarb3
• Reactivity: Mouse
• Synonyms: CD36/SR-B3; Mouse CD36/SR-B3 PicoKine ELISA Kit; Platelet glycoprotein 4; CD36 molecule (thrombospondin receptor); CD36 antigen; CD36 antigen (collagen type I receptor thrombospondin receptor); CD36 molecule; CHDS7; Cluster determinant 36; Fatty acid translocase; Glycoprotein IIIb; GP3B; GP4; GPIIIB; GPIV; Leukocyte differentiation antigen; CD36; PAS 4 protein; PAS IV; PASIV; Platelet collagen receptor; Platelet glycoprotein IV; SCARB3; Scavenger receptor class B member 3; Thrombospondin receptor; CD36 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CD36
• Sequence Length: 472

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse CD36/SR-B3 PicoKine ELISA Kit standard curve )

Related Product Information for CD36 elisa kit

Principle of the assay: mouse CD36 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD36 has been precoated onto 96-well plates. Standards(CHO, G30-K439) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD36 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD36 amount of sample captured in plate.
Background: CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

NCBI and Uniprot Product Information

• NCBI GI #: 729081
• NCBI GeneID: 12491
• UniProt Accession #: Q08857
• Molecular Weight: 52,698 Da
• NCBI Official Full Name: Platelet glycoprotein 4
• NCBI Official Synonym Full Names: CD36 antigen
• NCBI Official Symbol: Cd36
• NCBI Official Synonym Symbols: FAT; GPIV; Scarb3
• NCBI Protein Information: platelet glycoprotein 4; PAS-4; GPIIIB; PAS IV; glycoprotein IIIb; fatty acid translocase; platelet glycoprotein IV
• UniProt Protein Name: Platelet glycoprotein 4
• Protein Family: Platelet glycoprotein
• UniProt Gene Name: Cd36
• UniProt Synonym Gene Names: GPIIIB; GPIV
• UniProt Entry Name: CD36_MOUSE

Uniprot Description

CD36: Seems to have numerous potential physiological functions. Binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. May function as a cell adhesion molecule. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport. Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects. Defects in CD36 are the cause of platelet glycoprotein IV deficiency (PG4D)[MIM:608404]; also known as CD36 deficiency. Platelet glycoprotein IV deficiency can be divided into 2 subgroups. The type I phenotype is characterized by platelets and monocytes/macrophages exhibiting complete CD36 deficiency. The type II phenotype lacks the surface expression of CD36 in platelets, but expression in monocytes/macrophages is near normal. Genetic variations in CD36 are associated with susceptibility to coronary heart disease type 7 (CHDS7). Belongs to the CD36 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell adhesion

Cellular Component: Golgi apparatus; extracellular space; protein complex; cell surface; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; integral to membrane; caveola; lipid raft; membrane; apical part of cell; plasma membrane; intracellular; sarcolemma; external side of plasma membrane

Molecular Function: low-density lipoprotein receptor activity; protein binding; transforming growth factor beta binding; low-density lipoprotein binding; high-density lipoprotein binding; lipid binding

Biological Process: positive regulation of blood coagulation; positive regulation of interleukin-12 production; cGMP-mediated signaling; phagocytosis, recognition; pattern recognition receptor signaling pathway; negative regulation of transcription from RNA polymerase II promoter; signal transduction; fatty acid metabolic process; negative regulation of transcription factor import into nucleus; low density lipoprotein mediated signaling; sequestering of lipid; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; transport; positive regulation of cell-matrix adhesion; regulation of transcription from RNA polymerase II promoter in response to oxidative stress; cell adhesion; long-chain fatty acid transport; receptor-mediated endocytosis; cholesterol transport; positive regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of systemic arterial blood pressure; positive regulation of interleukin-6 production; long-chain fatty acid metabolic process; positive regulation of tumor necrosis factor production; negative regulation of angiogenesis; positive regulation of peptidyl-tyrosine phosphorylation; defense response to Gram-positive bacterium; triacylglycerol metabolic process; response to mechanical stimulus; lipoprotein transport; fatty acid oxidation; positive regulation of phagocytosis, engulfment; plasma membrane long-chain fatty acid transport; nitric oxide mediated signal transduction; apoptotic cell clearance; fatty acid transport; positive regulation of cytokine secretion