Rat CD14 molecule ELISA Kit | CD14 elisa kit

Rat soluble cluster of differentiation 14, sCD14 ELISA Kit

Cat. Num. AAA703387

• Reactivity: Rat
• Synonyms: CD14 molecule; Rat soluble cluster of differentiation 14; sCD14 ELISA Kit; CD14 antigen; monocyte differentiation antigen CD14; CD14 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat sCD14. No significant cross-reactivity or interference between rat sCD14 and analogues was observed.
• Sequence Length: 372

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 28 ng/ml -1800 ng/ml.
• Sensitivity: 7 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD14 elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD14 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126722873
• NCBI GeneID: 100008983
• NCBI Accession #: NP_001075664.1
• NCBI GenBank Nucleotide #: NM_001082195.2
• UniProt Accession #: Q28680
• Molecular Weight: 39,992 Da
• NCBI Official Full Name: monocyte differentiation antigen CD14
• NCBI Official Symbol: CD14
• NCBI Protein Information: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein
• UniProt Protein Name: Monocyte differentiation antigen CD14
• Protein Family: Monocyte differentiation antigen
• UniProt Gene Name: CD14
• UniProt Entry Name: CD14_RABIT

Uniprot Description

Function: In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules