Rat CCR2 (C-C chemokine receptor type 2) ELISA Kit | CCR2 elisa kit

Rat CCR2 (C-C chemokine receptor type 2) ELISA Kit

Cat. Num. AAA7606621

• Reactivity: Rat
• Synonyms: CCR2 (C-C chemokine receptor type 2); Rat CCR2 (C-C chemokine receptor type 2) ELISA Kit; CCR2 elisa kit

• Reactivity: Rat
• Sequence Length: 373

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.6-1000pg/ml
• Sensitivity: 3.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CCR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CCR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CCR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CCR2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 10719941
• NCBI GeneID: 60463
• UniProt Accession #: O55193
• Molecular Weight: 42,763 Da
• NCBI Official Full Name: C-C chemokine receptor type 2
• NCBI Official Synonym Full Names: C-C motif chemokine receptor 2
• NCBI Official Symbol: Ccr2
• NCBI Protein Information: C-C chemokine receptor type 2
• UniProt Protein Name: C-C chemokine receptor type 2
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: Ccr2
• UniProt Synonym Gene Names: Cmkbr2; C-C CKR-2; CC-CKR-2; CCR-2; CCR2

NCBI Description

mediates leukocyte migration and activation [RGD, Feb 2006]

Uniprot Description

CCR2: a seven transmembrane protein closely related to CCR1. Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Expressed at high levels in primary neutrophils and primary monocytes, and is further upregulated on neutrophil activation and during monocyte to macrophage differentiation. Transduces a signal by increasing the intracellular calcium ions level. Alternative co receptor with CD4 for HIV-1 infection. Two splice-variant isoforms have been described.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 8q32

Cellular Component: cell soma; cytoplasm; cytosol; dendrite; external side of plasma membrane; perikaryon; perinuclear region of cytoplasm; plasma membrane

Molecular Function: C-C chemokine binding; C-C chemokine receptor activity; CCR2 chemokine receptor binding; chemokine binding; cytokine binding; protein homodimerization activity

Biological Process: blood vessel remodeling; cellular calcium ion homeostasis; cellular defense response; cellular homeostasis; cytokine and chemokine mediated signaling pathway; G-protein coupled receptor protein signaling pathway; hemopoiesis; homeostasis of number of cells within a tissue; humoral immune response; immune response; inflammatory response; inflammatory response to antigenic stimulus; monocyte chemotaxis; negative regulation of angiogenesis; negative regulation of eosinophil degranulation; negative regulation of T-helper 2 type immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of inflammatory response; positive regulation of interferon-gamma production; positive regulation of interleukin-2 production; positive regulation of MAPKKK cascade; positive regulation of T cell activation; positive regulation of T-helper 1 type immune response; positive regulation of tumor necrosis factor biosynthetic process; positive regulation of tumor necrosis factor production; regulation of cell migration; response to hyperoxia; response to hypoxia; sensory perception of pain