Rat CXCR2 (C-X-C chemokine receptor type 2) ELISA Kit | CXCR2 elisa kit

Rat CXCR2 (C-X-C chemokine receptor type 2) ELISA Kit

Cat. Num. AAA7606600

• Gene Names: CXCR2; CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b
• Reactivity: Rat
• Synonyms: CXCR2 (C-X-C chemokine receptor type 2); Rat CXCR2 (C-X-C chemokine receptor type 2) ELISA Kit; IL8RB; CXC-R2; CXCR-2; CDw128b; GRO; MGSA receptor; High affinity interleukin-8 receptor B; IL-8R B; IL-8 receptor type 2; CD182;; CXCR2 elisa kit

• Reactivity: Rat
• Sequence Length: 360

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 46.875-3000pg/ml
• Sensitivity: 28.125pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CXCR2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- CXCR2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCR2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CXCR2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CXCR2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 269973859
• NCBI GeneID: 3579
• NCBI Accession #: NP_001161770.1
• NCBI GenBank Nucleotide #: NM_001168298.1
• UniProt Accession #: P25025; Q8IUZ1; Q9P2T6; Q9P2T7; AAB24742; AAA15417
• Molecular Weight: 40,759 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 2
• NCBI Official Synonym Full Names: C-X-C motif chemokine receptor 2
• NCBI Official Symbol: CXCR2
• NCBI Official Synonym Symbols: CD182; IL8R2; IL8RA; IL8RB; CMKAR2; CDw128b
• NCBI Protein Information: C-X-C chemokine receptor type 2
• UniProt Protein Name: C-X-C chemokine receptor type 2
• Protein Family: C-X-C chemokine receptor
• UniProt Gene Name: CXCR2
• UniProt Synonym Gene Names: IL8RB; CXC-R2; CXCR-2; IL-8R B

NCBI Description

The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009]

Uniprot Description

IL8RB: Receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Binds to IL-8 with high affinity. Also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; Receptor, cytokine

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: cell surface; integral to plasma membrane; intracellular; mast cell granule; membrane; plasma membrane; secretory granule membrane

Molecular Function: C-X-C chemokine receptor activity; interleukin-8 binding; interleukin-8 receptor activity; protein binding; signal transducer activity

Biological Process: cell surface receptor linked signal transduction; cellular defense response; chemotaxis; dendritic cell chemotaxis; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); inflammatory response; neutrophil activation; neutrophil chemotaxis; neutrophil degranulation; positive regulation of cell proliferation; receptor internalization; signal transduction