Typical Testing Data/Standard Curve (for reference only)
(Rat CCL4/MIP-1 beta PicoKine ELISA Kit standard curve )
Rat CCL4/MIP-1 beta ELISA Kit | CCL4 elisa kit
Rat CCL4/MIP-1 beta PicoKine ELISA Kit
Gene Names
Ccl4; Scya4; Mip1-b
Reactivity
Rat
Synonyms
CCL4/MIP-1 beta; Rat CCL4/MIP-1 beta PicoKine ELISA Kit; C-C motif chemokine 4; chemokine (C-C motif) ligand 4; ACT-2; ACT2; AT744.1; CC chemokine ligand 4; CCL4; CCL4_HUMAN; G-26 T-lymphocyte-secreted protein; HC21; LAG-1; LAG1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP-1-beta; MIP-1-beta(1-69); MIP-1-beta(3-69); MIP1-B; MIP1B; MIP1B1; PAT 744; Protein H400; SCYA2; SCYA4; SIS-gamma; Small-inducible cytokine A4; T-cell activation protein 2; CCL4 elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat CCL4
Sequence Length
92
Samples
Cell culture supernates, serum and plasma (heparin, EDTA, citrate)
Assay Type
Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat CCL4/MIP-1 beta PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat CCL4/MIP-1 beta PicoKine ELISA Kit standard curve )
Related Product Information for CCL4 elisa kit
Principle of the assay: rat CCL4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL4 has been precoated onto 96-well plates. Standards(E.Coli, A24-N92) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat CCL4 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).
Background: Chemokine (C-C motif) ligand 4, also known as CCL4, is a protein which in mouse is encoded by the CCL4 gene. It is a CC chemokine with specificity for CCR5 receptors. It is a chemoattractant for natural killer cells, monocytes and a variety of other immune cells. CCL4 is a major HIV-suppressive factor produced by CD8+ T cells. Performing-low memory CD8+ T cells that normally synthesize MIP-1-beta. By analysis of somatic cell hybrids and by in situ hybridization, the CCL4 gene was assigned to 17q21-q23. Based on functional analysis of MIP-1beta, one or more additional beta chemokine receptors that transduce MIP-1beta signals must also exist. MIP-1alpha and MIP-1beta have been shown to lack affinity for the erythrocyte surface chemokine receptor/Duffy antigen that binds many alpha as well as beta chemokines (11, 14).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
10,234 Da
NCBI Official Full Name
C-C motif chemokine 4
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 4
NCBI Official Symbol
Ccl4
NCBI Official Synonym Symbols
Scya4; Mip1-b
NCBI Protein Information
C-C motif chemokine 4; MIP-1-beta; small inducible cytokine A4; small-inducible cytokine A4; macrophage inflammatory protein 1-beta; macrophage inflammatory protein-1 beta
UniProt Protein Name
C-C motif chemokine 4
Protein Family
UniProt Gene Name
Ccl4
UniProt Synonym Gene Names
Mip1b; Scya4; MIP-1-beta
UniProt Entry Name
CCL4_RAT
NCBI Description
a cytokine that is up-regulated during the inflammatory response; may be involved in intrapulmonary recruitment of neutrophils [RGD, Feb 2006]
Uniprot Description
Monokine with inflammatory and chemokinetic properties.
Research Articles on CCL4
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Product Notes
The Rat CCL4 ccl4 (Catalog #AAA176559) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176559 ELISA Kit recognizes Rat CCL4. It is sometimes possible for the material contained within the vial of "CCL4/MIP-1 beta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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