Rat CB1R/CNR1 (Cannabinoid receptor 1) ELISA Kit | CB1R/CNR1 elisa kit

Rat CB1R/CNR1 (Cannabinoid receptor 1) ELISA Kit

Cat. Num. AAA7606742

• Gene Names: Cnr1; SKR6R
• Reactivity: Rat
• Synonyms: CB1R/CNR1 (Cannabinoid receptor 1); Rat CB1R/CNR1 (Cannabinoid receptor 1) ELISA Kit; Cannabinoid receptor 1; CB-R; CB1; Brain-type cannabinoid receptor; Skr6; Cnr1; CB1R/CNR1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CB1R/CNR1. No significant cross-reactivity or interference between CB1R/CNR1 and analogues was observed.
• Sequence Length: 473

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for CB1R/CNR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 6978673
• NCBI GeneID: 25248
• NCBI Accession #: NP_036916.1
• NCBI GenBank Nucleotide #: NM_012784.4
• UniProt Accession #: P20272
• Molecular Weight: 52,845 Da
• NCBI Official Full Name: cannabinoid receptor 1
• NCBI Official Synonym Full Names: cannabinoid receptor 1
• NCBI Official Symbol: Cnr1
• NCBI Official Synonym Symbols: SKR6R
• NCBI Protein Information: cannabinoid receptor 1
• UniProt Protein Name: Cannabinoid receptor 1
• UniProt Gene Name: Cnr1
• UniProt Synonym Gene Names: Skr6; CB-R; CB1

NCBI Description

G-protein coupled receptor for psychoactive cannabinoids; may mediate inhibition of NO production and participate in retrograde signaling by endocannabinoids [RGD, Feb 2006]

Uniprot Description

CNR1: Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. Belongs to the G-protein coupled receptor 1 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: actin cytoskeleton; axon; growth cone; intracellular membrane-bound organelle; lipid raft; plasma membrane; presynaptic membrane

Molecular Function: cannabinoid receptor activity; drug binding; protein binding

Biological Process: aging; axonal fasciculation; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; glucose homeostasis; learning and/or memory; maternal process involved in pregnancy; memory; negative regulation of action potential; negative regulation of blood pressure; negative regulation of dopamine secretion; negative regulation of fatty acid beta-oxidation; negative regulation of ion transport; negative regulation of mast cell activation; negative regulation of nitric-oxide synthase activity; positive regulation of acute inflammatory response to antigenic stimulus; positive regulation of apoptosis; positive regulation of blood pressure; positive regulation of fever; regulation of insulin secretion; regulation of lipid metabolic process; regulation of synaptic transmission, GABAergic; regulation of synaptic transmission, glutamatergic; response to cocaine; response to ethanol; response to lipopolysaccharide; response to morphine; response to nicotine; response to nutrient; sensory perception of pain; spermatogenesis