Typical Testing Data/Standard Curve (for reference only)
Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit | CAMK2g elisa kit
Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit
Reactivity
Rat
Synonyms
Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g); Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit; CAMK2g elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of CAMK2g. No significant cross-reactivity or interference between CAMK2g and analogues was observed.
Sequence Length
527
Samples
Tissue homogenates, cell lysates and other biological fluids.
Assay Type
Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.123ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CAMK2g were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CAMK2g were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CAMK2g elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CAMK2g in rat tissue homogenates, cell lysates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAMK2g. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CAMK2g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAMK2g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CAMK2g in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAMK2g. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CAMK2g. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CAMK2g, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CAMK2g in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,961 Da
NCBI Official Full Name
calcium/calmodulin-dependent protein kinase type II subunit gamma
NCBI Official Synonym Full Names
calcium/calmodulin-dependent protein kinase II gamma
NCBI Official Symbol
Camk2g
NCBI Protein Information
calcium/calmodulin-dependent protein kinase type II subunit gamma
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit gamma
Protein Family
UniProt Gene Name
Camk2g
UniProt Synonym Gene Names
CaM kinase II subunit gamma; CaMK-II subunit gamma
NCBI Description
gamma chain of the CaM kinase II; involved in regulation of potassium currents, particularly in response to oxidative stress [RGD, Feb 2006]
Uniprot Description
Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca2+/calmodulin-binding and autophosphorylation, and is involved in sarcoplasmic reticulum Ca2+ transport in skeletal muscle and may function in dendritic spine and synapse formation and neuronal plasticity. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca2+ transport and in fast-twitch muscle participates in the control of Ca2+ release from the SR through phosphorylation of the ryanodine receptor-coupling factor triadin. In neurons, may participate in the promotion of dendritic spine and synapse formation and maintenance of synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning ().
Research Articles on CAMK2g
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Product Notes
The Rat CAMK2g camk2g (Catalog #AAA451821) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA451821 ELISA Kit recognizes Rat CAMK2g. It is sometimes possible for the material contained within the vial of "Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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