Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit | CAMK2g elisa kit

Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit

Cat. Num. AAA2024075

• Reactivity: Rat
• Synonyms: Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g); Rat Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) ELISA Kit; CAMKII; CAMK2G; CAMK; CAMK-II; CAMKG; CaM Kinase; CaM kinase II subunit gamma; CaMK-II subunit gamma; CAMK2g elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g).; No significant cross-reactivity or interference between Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) and analogues was observed.
• Sequence Length: 495

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CAMK2g elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Gamma (CAMK2g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CAMK2g elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 21039158
• Molecular Weight: 55,961 Da
• NCBI Official Full Name: calcium/calmodulin-dependent protein kinase II gamma, partial
• UniProt Protein Name: Calcium/calmodulin-dependent protein kinase type II subunit gamma
• Protein Family: Calcium/calmodulin-dependent protein kinase
• UniProt Gene Name: CAMK2G
• UniProt Synonym Gene Names: CAMK; CAMK-II; CAMKG; CaM kinase II subunit gamma; CaMK-II subunit gamma
• UniProt Entry Name: KCC2G_HUMAN

Uniprot Description

CAMK2G: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; EC 2.7.11.17; CAMK group; CAMK2 family

Chromosomal Location of Human Ortholog: 10q22

Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; membrane; plasma membrane; cytosol

Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; calcium-dependent protein serine/threonine phosphatase activity; calmodulin-dependent protein kinase activity; ATP binding

Biological Process: nervous system development; synaptic transmission; dephosphorylation; regulation of skeletal muscle adaptation; insulin secretion; protein amino acid autophosphorylation; calcium ion transport; cytokine and chemokine mediated signaling pathway; regulation of calcium ion transport; cell differentiation; protein oligomerization; G1/S transition of mitotic cell cycle