Typical Standard Curve/Testing Data
Rat Calcium-binding and spermatid-specific protein 1 (C4orf35) ELISA Kit | C4orf35 elisa kit
Rat Calcium-binding and spermatid-specific protein 1 (C4orf35) ELISA Kit
Gene Names
CABS1; CLPH; C4orf35; NYD-SP26
Reactivity
Rat
Synonyms
Calcium-binding and spermatid-specific protein 1 (C4orf35); Rat Calcium-binding and spermatid-specific protein 1 (C4orf35) ELISA Kit; C4orf35 elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Related Product Information for C4orf35 elisa kit
Intended Uses: This C4orf35 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat C4orf35. This ELISA kit for research use only!
Principle of the Assay: C4orf35 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-C4orf35 antibody and an C4orf35-HRP conjugate. The assay sample and buffer are incubated together with C4orf35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4orf35 concentration since C4orf35 from samples and C4orf35-HRP conjugate compete for the anti-C4orf35 antibody binding site. Since the number of sites is limited, as more sites are occupied by C4orf35 from the sample, fewer sites are left to bind C4orf35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4orf35 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: C4orf35 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-C4orf35 antibody and an C4orf35-HRP conjugate. The assay sample and buffer are incubated together with C4orf35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the C4orf35 concentration since C4orf35 from samples and C4orf35-HRP conjugate compete for the anti-C4orf35 antibody binding site. Since the number of sites is limited, as more sites are occupied by C4orf35 from the sample, fewer sites are left to bind C4orf35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C4orf35 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for C4orf35 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43,003 Da
NCBI Official Full Name
calcium-binding and spermatid-specific protein 1
NCBI Official Synonym Full Names
calcium-binding protein, spermatid-specific 1
NCBI Official Symbol
CABS1
NCBI Official Synonym Symbols
CLPH; C4orf35; NYD-SP26
NCBI Protein Information
calcium-binding and spermatid-specific protein 1; casein-like phosphoprotein; testis development protein NYD-SP26; calcium-binding protein, sperm-specific 1
UniProt Protein Name
Calcium-binding and spermatid-specific protein 1
UniProt Gene Name
CABS1
UniProt Synonym Gene Names
C4orf35
UniProt Entry Name
CABS1_HUMAN
Uniprot Description
CLPH:
Protein type: Calcium-binding; Mitochondrial
Chromosomal Location of Human Ortholog: 4q13.3
Cellular Component: mitochondrial inner membrane
Molecular Function: calcium ion binding
Biological Process: spermatogenesis
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Product Notes
The Rat C4orf35 cabs1 (Catalog #AAA7227393) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7227393 ELISA Kit recognizes Rat C4orf35. It is sometimes possible for the material contained within the vial of "Calcium-binding and spermatid-specific protein 1 (C4orf35), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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