Rat Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit | CELSR2 elisa kit

Rat Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit

Cat. Num. AAA452565

• Gene Names: Celsr2; Megf3
• Reactivity: Rat
• Synonyms: Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2); Rat Cadherin EGF LAG Seven Pass G-Type Receptor 2 (CELSR2) ELISA Kit; CDHF10 EGFL2 Flamingo 1 MEGF3 Cadherin family member 10 Epidermal growth factor-like protein 2 Multiple epidermal growth factor-like domains protein 3; CELSR2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CELSR2. No significant cross-reactivity or interference between CELSR2 and analogues was observed.
• Sequence Length: 2144

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.24ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CELSR2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CELSR2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CELSR2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CELSR2 in rat tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CELSR2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CELSR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CELSR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CELSR2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 22095545
• NCBI GeneID: 83465
• UniProt Accession #: Q9QYP2
• Molecular Weight: 233,481 Da
• NCBI Official Full Name: Cadherin EGF LAG seven-pass G-type receptor 2
• NCBI Official Synonym Full Names: cadherin, EGF LAG seven-pass G-type receptor 2
• NCBI Official Symbol: Celsr2
• NCBI Official Synonym Symbols: Megf3
• NCBI Protein Information: cadherin EGF LAG seven-pass G-type receptor 2
• UniProt Protein Name: Cadherin EGF LAG seven-pass G-type receptor 2
• Protein Family: Cadherin EGF LAG seven-pass G-type receptor
• UniProt Gene Name: Celsr2
• UniProt Synonym Gene Names: Megf3; Multiple EGF-like domains protein 3

NCBI Description

cell-cell adhesion molecule [RGD, Feb 2006]

Uniprot Description

Receptor that may have an important role in cell/cell signaling during nervous system formation.