Western Blot (WB)
(Figure 1. Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
Mouse MLH1 Antibody | anti-MLH1 antibody
MLH1 Antibody
IHC: 1:200-1:1000
IF: 1:200-1:1000
ELISA: 1:10000
Western Blot (WB)
(Figure 1. Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
Western Blot (WB)
(Figure 1. Western blot analysis using MLH1 mouse mAb against Hela (1), MCF-7 (2) and A549 (3), Jurkat (4), 2R75 (5) and COS (6) cell lysate.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemical analysis of paraffin-embedded human rectum cancer (left) and ovarian cancer (right) tissues, showing nuclear localization with DAB staining using MLH1 mouse mAb.)
Immunofluorescence (IF)
(Figure 3. Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
Immunofluorescence (IF)
(Figure 3. Confocal immunofluorescence analysis of Hela cells using MLH1 mouse mAb (green), showing nuclear localization. Red. Actin filaments have been labeled with Alexa Fluor-555 phalloidin.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]
Uniprot Description
Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1) through phosphorylation and activation of MAP2K4/MKK4 and MAP2K7/MKK7. Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
Research Articles on MLH1
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Product Notes
The MLH1 map3k11 (Catalog #AAA850685) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MLH1 Antibody reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's MLH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:200-1:1000 IF: 1:200-1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the MLH1 map3k11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MLH1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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