Rat C4 Binding Protein Alpha (C4BPa) ELISA Kit | C4BPa elisa kit

Rat C4 Binding Protein Alpha (C4BPa) ELISA Kit

Cat. Num. AAA2703499

• Gene Names: C4bpa; C4BP
• Reactivity: Rat
• Synonyms: C4 Binding Protein Alpha (C4BPa); Rat C4 Binding Protein Alpha (C4BPa) ELISA Kit; C4BP-a; PRP; C4BP; Complement Component 4 Binding Protein; Alpha; Proline-rich protein; C4BPa elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of C4 Binding Protein Alpha (C4BPa).; No significant cross-reactivity or interference between C4 Binding Protein Alpha (C4BPa) and analogues was observed.
• Sequence Length: 601

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.65ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level C4 Binding Protein Alpha (C4BPa) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level C4 Binding Protein Alpha (C4BPa) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for C4BPa elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to C4 Binding Protein Alpha (C4BPa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to C4 Binding Protein Alpha (C4BPa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C4 Binding Protein Alpha (C4BPa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of C4 Binding Protein Alpha (C4BPa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 77539456
• NCBI GeneID: 24235
• NCBI Accession #: NP_036648.2
• NCBI GenBank Nucleotide #: NM_012516.2
• Molecular Weight: 62,266 Da
• NCBI Official Full Name: C4b-binding protein alpha chain
• NCBI Official Synonym Full Names: complement component 4 binding protein, alpha
• NCBI Official Symbol: C4bpa
• NCBI Official Synonym Symbols: C4BP
• NCBI Protein Information: C4b-binding protein alpha chain
• UniProt Protein Name: C4b-binding protein alpha chain
• Protein Family: C4b-binding protein
• UniProt Gene Name: C4bpa
• UniProt Synonym Gene Names: C4bp

NCBI Description

regulatory protein for the complement system of the innate immune response [RGD, Feb 2006]

Uniprot Description

Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. Alpha chain binds C4b. It interacts also with anticoagulant protein S and with serum amyloid P component.