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Rat C-X-C motif chemokine 2 (CXCL2) ELISA Kit | CXCL2 elisa kit

Rat C-X-C motif chemokine 2 (CXCL2) ELISA Kit

Gene Names
Cxcl2; Mip-2; Scyb2
Reactivity
Rat
Synonyms
C-X-C motif chemokine 2 (CXCL2); Rat C-X-C motif chemokine 2 (CXCL2) ELISA Kit; CINC-2a; GRO2; GROb; MGSA-b; MIP-2a; MIP2; MIP2A; SCYB2; GRO2 oncogene|MGSA beta|melanoma growth stimulatory activity beta; CXCL2 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat CXCL2. No significant cross-reactivity or interference between Rat CXCL2 and analogues was observed.
Assay Type
Sandwich
Assay
Serum, Plasma, Other biological fluids
Analysis Method
Quantitive
Detection Wavelength
450nm
Sample Volume
1-200uL
Assay Duration
1-4.5h
Precision
Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Test Principle
This assay employs a two-site sandwich ELISA to quantitate CXCL2 in samples. An antibody specific for CXCL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CXCL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CXCL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CXCL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Preparation and Storage
Store at 2-8 degree C. Please refer to Instruction Manual.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,783 Da
NCBI Official Full Name
C-X-C motif chemokine 2
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 2
NCBI Official Symbol
Cxcl2
NCBI Official Synonym Symbols
Mip-2; Scyb2
NCBI Protein Information
C-X-C motif chemokine 2; CINC-3; MIP2; cytokine-induced neutrophil chemoattractant 3; macrophage inflammatory protein 2; small inducible cytokine subfamily, member 2
UniProt Protein Name
C-X-C motif chemokine 2
Protein Family
UniProt Gene Name
Cxcl2
UniProt Synonym Gene Names
Cinc3; Mip-2; Mip2; Scyb2; CINC-3; MIP2
UniProt Entry Name
CXCL2_RAT

NCBI Description

chemokine involved in the pulmonary inflammatory response [RGD, Feb 2006]

Uniprot Description

CXCL3: Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. In vitro, the processed form GRO-gamma(5-73) shows a fivefold higher chemotactic activity for neutrophilic granulocytes. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: extracellular space

Molecular Function: chemokine activity; CXCR chemokine receptor binding

Biological Process: neutrophil chemotaxis; wound healing; leukocyte chemotaxis; response to glucocorticoid stimulus; response to amphetamine; positive regulation of leukocyte chemotaxis; response to estradiol stimulus; regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; response to heat; response to molecule of bacterial origin; response to gamma radiation; immune response; inflammatory response

Research Articles on CXCL2

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Product Notes

The Rat CXCL2 cxcl2 (Catalog #AAA2803236) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2803236 ELISA Kit recognizes Rat CXCL2. It is sometimes possible for the material contained within the vial of "C-X-C motif chemokine 2 (CXCL2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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