Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat C-type natriuretic peptide (CNP) Standard Curve.)
Rat C-Type Natriuretic Peptide (CNP) ELISA Kit | CNP elisa kit
Rat C-Type Natriuretic Peptide (CNP) ELISA Kit
Reactivity
Rat
Synonyms
C-Type Natriuretic Peptide (CNP); Rat C-Type Natriuretic Peptide (CNP) ELISA Kit; NPPC; CNP; OTTHUMP00000203815; CNP elisa kit
Reactivity
Rat
Specificity
Rat C-type natriuretic peptide (CNP) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NPPC. No significant cross-reactivity or interference between Rat NPPC and analogues was observed.
Sequence Length
126
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat C-type natriuretic peptide (CNP) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat C-type natriuretic peptide (CNP) Standard Curve.)
Related Product Information for CNP elisa kit
Description: This Rat C-type natriuretic peptide (CNP) ELISA Kit employs a two-site sandwich ELISA to quantitate NPPC in samples. An antibody specific for NPPC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NPPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NPPC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NPPC bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: C-type natriuretic peptide (CNP), the third member of the natriuretic peptide family, has been found at its highest tissue concentrations in the anterior pituitary, where it is localised in gonadotrophs. Its specific guanylyl cyclase-containing receptor, GC-B, is also expressed on several anterior pituitary cell types, and CNP potently stimulates cGMP accumulation in rat pituitary cell cultures and pituitary cell lines. The mouse gonadotroph-derived alpha T3-1 cell line has been shown to express CNP as well as GC-B (but not GC-A) receptors, suggesting that CNP may well be an autocrine regulator of gonadotrophs. Protein kinase C activation with phorbol esters also inhibited CNP-stimulated cGMP accumulation and such inhibition was also seen in cells desensitised by pretreatment with CNP. Thus it appears that the endogenous GC-B receptors of alpha T3-1 cells are subject to both homologous and heterologous desensitisation, that the mechanisms underlying these forms of desensitisation are distinct, and that cGMP elevation alone is insufficient to desensitise GC-B receptors.
Background: C-type natriuretic peptide (CNP), the third member of the natriuretic peptide family, has been found at its highest tissue concentrations in the anterior pituitary, where it is localised in gonadotrophs. Its specific guanylyl cyclase-containing receptor, GC-B, is also expressed on several anterior pituitary cell types, and CNP potently stimulates cGMP accumulation in rat pituitary cell cultures and pituitary cell lines. The mouse gonadotroph-derived alpha T3-1 cell line has been shown to express CNP as well as GC-B (but not GC-A) receptors, suggesting that CNP may well be an autocrine regulator of gonadotrophs. Protein kinase C activation with phorbol esters also inhibited CNP-stimulated cGMP accumulation and such inhibition was also seen in cells desensitised by pretreatment with CNP. Thus it appears that the endogenous GC-B receptors of alpha T3-1 cells are subject to both homologous and heterologous desensitisation, that the mechanisms underlying these forms of desensitisation are distinct, and that cGMP elevation alone is insufficient to desensitise GC-B receptors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
C-type natriuretic peptide
NCBI Official Synonym Full Names
natriuretic peptide C
NCBI Official Symbol
Nppc
NCBI Protein Information
C-type natriuretic peptide
UniProt Protein Name
C-type natriuretic peptide
Protein Family
UniProt Gene Name
Nppc
UniProt Synonym Gene Names
Cnp
NCBI Description
binds natriuretic peptide receptor; involved in inhibition of growth and proliferation in vascular smooth muscle cells [RGD, Feb 2006]
Uniprot Description
Hormone which plays a role in endochondral ossification through regulation of cartilaginous growth plate chondrocytes proliferation and differentiation. May also be vasoactive and natriuretic. Specifically binds and stimulates the cGMP production of the NPR2 receptor. Binds the clearance receptor NPR3 ().
Research Articles on CNP
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Product Notes
The Rat CNP nppc (Catalog #AAA9712501) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712501 ELISA Kit recognizes Rat CNP. It is sometimes possible for the material contained within the vial of "C-Type Natriuretic Peptide (CNP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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