ELISA (EIA)
(O8075-07A used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin. Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p)
ELISA (EIA)
(O8075-07A used in an ELISA assay for the measurement of ovalbumin specific IgG in an experimental mouse model of airway inflammation. Basal and OVA-specific IgE levels are increased in p110y/d-/- mice. Total and OVA-specific IgE was determined in sera of PBS-treated and OVA-treated KO and corresponding WT mice using ELISA. (A) Total serum IgE. (B) OVA-specific serum IgE. Bars express means + SD; n = 4-8 pooled from three independent experiments. Data were analysed by One-way ANOVA followed by Bonferroni's comparison tests for selected pairs of columns; **P)