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ELISA (EIA) (O8075-07A used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin. Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p)

Mouse Ovalbumin Monoclonal Antibody

Ovalbumin (FITC)

Applications
FLISA
Purity
Purified from tissue culture supernatant.
Synonyms
Ovalbumin; Monoclonal Antibody; Ovalbumin (FITC); anti-Ovalbumin antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgE
Clone Number
10F254
Specificity
Recognizes ovalbumin (OVA). Suitable for use as a mouse IgE standard in ELISA assays.
Purity/Purification
Purified from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Applicable Applications for anti-Ovalbumin antibody
FLISA
Immunogen
Ovalbumin
Conjugate
FITC
Preparation and Storage
Store at -20 degree C.

ELISA (EIA)

(O8075-07A used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin. Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p)

ELISA (EIA) (O8075-07A used to generate a standard curve for murine IgE in a sandwich ELISA detected using biotin conjugated ovalbumin and streptavidin. Analysis of OVA-specific IgE. The levels of IgE in sera obtained before, during, and after ASIT were detected and quantified by ELISA. The results are presented as mean IgE concentration (mean±SE, n = 8 for each). AD without ASIT: OVA-sensitized untreated mice; ASIT with OVA: OVA-sensitized mice treated by ASIT with OVA; ASIT with sOVA: OVA-sensitized mice treated by ASIT with sOVA; placebo: PBS-sensitized mice. (*, p)

ELISA (EIA)

(O8075-07A used in an ELISA assay for the measurement of ovalbumin specific IgG in an experimental mouse model of airway inflammation. Basal and OVA-specific IgE levels are increased in p110y/d-/- mice. Total and OVA-specific IgE was determined in sera of PBS-treated and OVA-treated KO and corresponding WT mice using ELISA. (A) Total serum IgE. (B) OVA-specific serum IgE. Bars express means + SD; n = 4-8 pooled from three independent experiments. Data were analysed by One-way ANOVA followed by Bonferroni's comparison tests for selected pairs of columns; **P)

ELISA (EIA) (O8075-07A used in an ELISA assay for the measurement of ovalbumin specific IgG in an experimental mouse model of airway inflammation. Basal and OVA-specific IgE levels are increased in p110y/d-/- mice. Total and OVA-specific IgE was determined in sera of PBS-treated and OVA-treated KO and corresponding WT mice using ELISA. (A) Total serum IgE. (B) OVA-specific serum IgE. Bars express means + SD; n = 4-8 pooled from three independent experiments. Data were analysed by One-way ANOVA followed by Bonferroni's comparison tests for selected pairs of columns; **P)
Product Categories/Family for anti-Ovalbumin antibody
References
1. Stevens, T. et al. (2008) Increased transcription of immune and metabolic pathways in naive and allergic mice exposed to diesel exhaust. Toxicol Sci. 102: 359-70. 2. Fairley,K.J. et al. (2007) Exposure to the immunosuppressant, perfluorooctanoic acid, enhances the murine IgE and airway hyperreactivity response to ovalbumin. Toxicol.Sci. 97: 375-83 3. Ellertsen,L.K. et al. (2010) Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice. BMC Immunol. 11: 8-174. Kambayashi, T. et al. (2008) Indirect involvement of allergen-captured mast cells in antigen presentation. Blood. 111:1489-96. 5. Paliwal, S. et al. (2010) One-step acquisition of functional biomolecules from tissues. Proc Natl Acad Sci U S A. 107: 14627-32. 6. Chida, Y. et al. (2007) Early-life psychological stress exacerbates adult mouse asthma via the hypothalamus-pituitary-adrenal axis. Am J Respir Crit Care Med. 175: 316-22. 7. Suzaki, Y. et al. (2007) A small-molecule compound targeting CCR5 and CXCR3 prevents airway hyperresponsiveness and inflammation. Eur Respir J. 31: 783-9. 8. Suzaki, Y. et al. (2005) A potent antiangiogenic factor, endostatin prevents the development of asthma in a murine model. J Allergy Clin Immunol. 116 (6): 1220-7. 9. Hansen, J.S. et al. (2011) Determinants of experimental allergic responses: interactions between allergen dose, sex and age. Scand J Immunol. 73 (6): 554-67. 10. Nygaard, U.C. et al. (2015) Early life exposure to bisphenol A investigated in mouse models of airway allergy, food allergy and oral tolerance. Food Chem Toxicol. 83: 17-25. 11. Shershakova N et al. (2015) Allergen-Specific Immunotherapy with Monomeric Allergoid in a Mouse Model of Atopic Dermatitis. PLoS One. 10 (8): e0135070. 13. Diesner, S.C. et al. (2016) A distinct microbiota composition is associated with protection from food allergy in an oral mouse immunization model. Clin Immunol. pii: S1521-6616(16)30300-X. 14. Cheung DS et al. (2010) Development of atopy by severe paramyxoviral infection in a mouse model. Ann Allergy Asthma Immunol. 105 (6): 437-443.e1. 15. Garbani, M. et al. (2016) Allergen-loaded strontium-doped hydroxyapatite spheres improve allergen-specific immunotherapy in mice. Allergy. Sep 3. 16. Mothes, B. et al. (2016) p110y/d Double-Deficiency Induces Eosinophilia and IgE Production but Protects from OVA-Induced Airway Inflammation. PLoS One. 11 (7): e0159310. 18. Andreassen, M. et al. (2015) Cry1Ab protein from Bacillus thuringiensis and MON810 cry1Ab-transgenic maize exerts no adjuvant effect after airway exposure. Scand J Immunol. 81 (3): 192-200. 19. Hamada. K, et al. (2003) Allergen-independent maternal transmission of asthma susceptibility. J. Immunol. 170: 1683-1689.

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Product Notes

The Ovalbumin (Catalog #AAA6250121) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ovalbumin can be used in a range of immunoassay formats including, but not limited to, FLISA. Researchers should empirically determine the suitability of the Ovalbumin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ovalbumin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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