Rat Butyrylcholinesterase (BCHE) ELISA Kit | BCHE elisa kit

Rat Butyrylcholinesterase (BCHE) ELISA Kit

Cat. Num. AAA2088166

• Reactivity: Rat
• Synonyms: Butyrylcholinesterase (BCHE); Rat Butyrylcholinesterase (BCHE) ELISA Kit; BuChE; CHE1; E1; Pseudocholinesterase; Cholinesterase; Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; BCHE elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Butyrylcholinesterase (BCHE).; No significant cross-reactivity or interference between Butyrylcholinesterase (BCHE) and analogues was observed.
• Sequence Length: 597

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.63ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Butyrylcholinesterase (BCHE) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Butyrylcholinesterase (BCHE) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BCHE elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Butyrylcholinesterase (BCHE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Butyrylcholinesterase (BCHE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Butyrylcholinesterase (BCHE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Butyrylcholinesterase (BCHE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for BCHE elisa kit

Enzyme & Kinase; Metabolic pathway; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 12621110
• NCBI GeneID: 65036
• NCBI Accession #: NP_075231.1
• NCBI GenBank Nucleotide #: NM_022942.1
• UniProt Accession #: Q9JKC1; F1LQK0
• Molecular Weight: 67,777 Da
• NCBI Official Full Name: cholinesterase
• NCBI Official Synonym Full Names: butyrylcholinesterase
• NCBI Official Symbol: Bche
• NCBI Protein Information: cholinesterase
• UniProt Protein Name: Carboxylic ester hydrolase
• Protein Family: Cholinesterase
• UniProt Gene Name: Bche

Uniprot Description

BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family.

Protein type: EC 3.1.1.8; Hydrolase; Nuclear envelope; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q32

Cellular Component: endoplasmic reticulum; endoplasmic reticulum lumen; extracellular space; membrane; nuclear envelope lumen

Molecular Function: acetylcholinesterase activity; choline binding; cholinesterase activity; identical protein binding

Biological Process: choline metabolic process; learning; negative regulation of cell proliferation; negative regulation of synaptic transmission; neuroblast differentiation; response to alkaloid; response to drug; response to folic acid; response to glucocorticoid stimulus; response to nutrient