Rat Breast cancer susceptibility protein 1, BRCA-1 ELISA Kit | BRCA-1 elisa kit

Rat Breast cancer susceptibility protein 1, BRCA-1 ELISA Kit

Cat. Num. AAA166241

• Gene Names: BRAP; IMP; BRAP2; RNF52
• Reactivity: Rat
• Synonyms: Breast cancer susceptibility protein 1; BRCA-1; Rat Breast cancer susceptibility protein 1; BRCA-1 ELISA Kit; BRCA-1 elisa kit

• Reactivity: Rat
• Sequence Length: 592

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15ng/L - 3000ng/L
• Sensitivity: 7.52ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BRCA-1 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Breast Cancer Susceptibility Protein 1 (also known as BRCA-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat BRCA-1 antibody. BRCA-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat BRCA-1 Antibody is added and binds to BRCA-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BRCA-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat BRCA-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 188497705
• NCBI GeneID: 8315
• NCBI Accession #: NP_006759.3
• NCBI GenBank Nucleotide #: NM_006768.4
• UniProt Accession #: Q7Z569; O43238; O75341; B4DRM1; B9EGS8
• Molecular Weight: 47,853 Da
• NCBI Official Full Name: BRCA1-associated protein
• NCBI Official Synonym Full Names: BRCA1 associated protein
• NCBI Official Symbol: BRAP
• NCBI Official Synonym Symbols: IMP; BRAP2; RNF52
• NCBI Protein Information: BRCA1-associated protein
• UniProt Protein Name: BRCA1-associated protein
• UniProt Gene Name: BRAP
• UniProt Synonym Gene Names: IMP

NCBI Description

The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008]

Uniprot Description

Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.