Rabbit anti-Human CCL25 Polyclonal Antibody | anti-CCL25 antibody

CCL25 Polyclonal Antibody

Cat. Num. AAA3008104

• Gene Names: CCL25; TECK; Ckb15; SCYA25
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CCL25; Polyclonal Antibody; CCL25 Polyclonal Antibody; C-C motif chemokine 25; Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine; SCYA25; TECK; anti-CCL25 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CCL25 polyclonal antibody detects endogenous levels of CCL25 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 150

• Applicable Applications for anti-CCL25 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human CCL25.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using CCL25 antibody at dilution of 1:100 (40x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using CCL25 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-CCL25 antibody

Chemokines are likely to play an important role in regulating the trafficking of developing T cells within the thymus. Chemokine C-C thymus expressed chemokine (TECK), also designated chemokine ligand 25 (CCL25), small induc-ible cytokine A25, chemokine b-15 or CK b-15, is expressed predominantly in thymic dendritic cells, thymic epithelial cells and in the small intestine. TECK, a CCR9 ligand, has suppressive activity against immature subsets of myeloid progenitors which have been stimulated to proliferate by multiple growth factors. TECK delivers signals through CCR9, which is important for the nav-igation of developing thymocytes. Bone marrow pre-pro-B cells and cells capable of generating pro-B colonies in the presence of interleukin-7 and Flt-3 ligand migrate to TECK, a response lost in later stages of B cell development.

NCBI and Uniprot Product Information

• NCBI GI #: 212286066
• NCBI GeneID: 6370
• UniProt Accession #: O15444
• Molecular Weight: ~ 17kDa
• NCBI Official Full Name: C-C motif chemokine 25
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 25
• NCBI Official Symbol: CCL25
• NCBI Official Synonym Symbols: TECK; Ckb15; SCYA25
• NCBI Protein Information: C-C motif chemokine 25
• UniProt Protein Name: C-C motif chemokine 25
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL25
• UniProt Synonym Gene Names: SCYA25; TECK
• UniProt Entry Name: CCL25_HUMAN

NCBI Description

This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extracellular region

Molecular Function: chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; immune response; chemotaxis; positive regulation of cell-matrix adhesion; inflammatory response; leukocyte migration