BACE2 / Beta secretase 2 Recombinant Protein | BACE2 recombinant protein

Recombinant Mouse BACE2 / Beta secretase 2 Protein (His tag)

Cat. Num. AAA2546685

• Gene Names: Bace2; ARP1; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: BACE2 / Beta secretase 2; Recombinant Mouse BACE2 / Beta secretase 2 Protein (His tag); BACE2; BACE2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 514

• Application Notes: The recombinant mouse BACE2 comprises 454 amino acids and has a predicted molecular mass of 49.2 kDa. The apparent molecular mass of the protein is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Predicted N Terminal: Ala 20
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for BACE2 recombinant protein

Background: BACE2, also known as beta secretase 2, belongs to the peptidase A1 family. It is a protease known to be an important enzyme involved in the cellular pathways. BACE2 has been shown to interact with GGA1 and GGA2. It is the major beta-secretase in vivo. BACE2 is located on chromosome 21 and may play a role in alzheimer's disease pathogenesis in down syndrome (DS). Overexpression of BACE2 by lentivirus markedly reduced amyloid beta protein production in primary neurons. Despite an extra copy of the BACE2 gene in DS and the increase of its transcription, BACE2 protein levels are unchanged.

Description: A DNA sequence encoding the mouse BACE2 (Q9JL18) (Met1-Pro462) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 244798416
• NCBI GeneID: 56175
• NCBI Accession #: NP_062390.3
• NCBI GenBank Nucleotide #: NM_019517.5
• UniProt Accession #: Q9JL18; Q3TNS7; Q8C5E9; Q8C793; Q9R1P7
• Molecular Weight: 55,800 Da
• NCBI Official Full Name: beta-secretase 2 preproprotein
• NCBI Official Synonym Full Names: beta-site APP-cleaving enzyme 2
• NCBI Official Symbol: Bace2
• NCBI Official Synonym Symbols: ARP1; ASP1; BAE2; DRAP; AEPLC; ALP56; ASP21; CDA13; CEAP1; AI850424; 1110059C24Rik
• NCBI Protein Information: beta-secretase 2
• UniProt Protein Name: Beta-secretase 2
• Protein Family: Beta-secretase
• UniProt Gene Name: Bace2
• UniProt Synonym Gene Names: ASP1; Asp 1; Beta-site APP cleaving enzyme 2

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. The encoded preproprotein undergoes proteolytic processing to generate an active endopeptidase enzyme. This transmembrane protease catalyzes the proteolysis of amyloid precursor protein to produce amyloid beta peptide. Mice lacking the encoded product exhibit increased pancreatic beta cell mass and improved glucose tolerance due to increased insulin secretion. [provided by RefSeq, Jul 2016]

Uniprot Description

BACE2: Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C- terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Belongs to the peptidase A1 family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.23.45; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 16|16 C4

Cellular Component: Golgi apparatus

Molecular Function: aspartic-type endopeptidase activity; beta-amyloid binding

Biological Process: beta-amyloid metabolic process; membrane protein ectodomain proteolysis; negative regulation of amyloid precursor protein biosynthetic process; protein catabolic process