Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Atrial natriuretic peptide-converting enzyme (CORIN) Standard Curve.)
Rat Atrial Natriuretic Peptide-Converting Enzyme (CORIN) ELISA Kit | CORIN elisa kit
Rat Atrial Natriuretic Peptide-Converting Enzyme (CORIN) ELISA Kit
Reactivity
Rat
Synonyms
Atrial Natriuretic Peptide-Converting Enzyme (CORIN); Rat Atrial Natriuretic Peptide-Converting Enzyme (CORIN) ELISA Kit; CORIN; ATC2; CRN; Lrp4; MGC119742; TMPRSS10; atrial natriuteric peptide-converting enzyme; corin; serine protease; heart specific serine proteinase; pro-ANP-convertase; pro-ANP-converting enzyme; CORIN elisa kit
Reactivity
Rat
Specificity
Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat CORIN. No significant cross-reactivity or interference between Rat CORIN and analogues was observed.
Sequence Length
1111
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Atrial natriuretic peptide-converting enzyme (CORIN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Atrial natriuretic peptide-converting enzyme (CORIN) Standard Curve.)
Related Product Information for CORIN elisa kit
Description: This Rat Atrial natriuretic peptide-converting enzyme (CORIN) ELISA Kit employs a two-site sandwich ELISA to quantitate CORIN in samples. An antibody specific for CORIN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CORIN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CORIN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CORIN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Corin encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase.The corin cDNA encodes a deduced 1,042-amino acid, type II transmembrane protein. At its N terminus, corin contains a short cytoplasmic domain and a transmembrane sequence. In the extracellular region of corin, there are 2 frizzled-like cysteine-rich motifs, 7 low density lipoprotein receptor repeats, a macrophage scavenger receptor-like domain, and a trypsin-like protease domain at the C terminus.
Background: Corin encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase.The corin cDNA encodes a deduced 1,042-amino acid, type II transmembrane protein. At its N terminus, corin contains a short cytoplasmic domain and a transmembrane sequence. In the extracellular region of corin, there are 2 frizzled-like cysteine-rich motifs, 7 low density lipoprotein receptor repeats, a macrophage scavenger receptor-like domain, and a trypsin-like protease domain at the C terminus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
atrial natriuretic peptide-converting enzyme
NCBI Official Synonym Full Names
corin, serine peptidase
NCBI Official Symbol
Corin
NCBI Protein Information
atrial natriuretic peptide-converting enzyme
UniProt Protein Name
Atrial natriuretic peptide-converting enzyme
Protein Family
UniProt Gene Name
Corin
Uniprot Description
Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide.
Research Articles on CORIN
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Product Notes
The Rat CORIN corin (Catalog #AAA9712589) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712589 ELISA Kit recognizes Rat CORIN. It is sometimes possible for the material contained within the vial of "Atrial Natriuretic Peptide-Converting Enzyme (CORIN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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