Rat Argininosuccinate lyase (ASL) ELISA Kit | ASL elisa kit

Rat Argininosuccinate lyase (ASL) ELISA Kit

Cat. Num. AAA7228548

• Reactivity: Rat
• Synonyms: Argininosuccinate lyase (ASL); Rat Argininosuccinate lyase (ASL) ELISA Kit; ASL elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 100-2500pg/mL
• Sensitivity: 1.0pg/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for ASL elisa kit

Intended Uses: This ASL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ASL. This ELISA kit for research use only!

Principle of the Assay||ASL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ASL antibody and an ASL-HRP conjugate. The assay sample and buffer are incubated together with ASL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASL concentration since ASL from samples and ASL-HRP conjugate compete for the anti-ASL antibody binding site. Since the number of sites is limited, as more sites are occupied by ASL from the sample, fewer sites are left to bind ASL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASL concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31377525
• NCBI GeneID: 59085
• NCBI Accession #: NP_067588.2
• NCBI GenBank Nucleotide #: NM_021577.3
• Molecular Weight: 51,550 Da
• NCBI Official Full Name: argininosuccinate lyase
• NCBI Official Synonym Full Names: argininosuccinate lyase
• NCBI Official Symbol: Asl
• NCBI Protein Information: argininosuccinate lyase; ASAL; arginosuccinase
• UniProt Protein Name: Argininosuccinate lyase
• UniProt Gene Name: Asl
• UniProt Synonym Gene Names: ASAL
• UniProt Entry Name: ARLY_RAT

Uniprot Description

ASL: Defects in ASL are the cause of arginosuccinic aciduria (ARGINSA). An autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness. Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.

Protein type: Lyase; Endoplasmic reticulum; Enzyme, misc.; Mitochondrial; EC 4.3.2.1; Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline

Cellular Component: mitochondrial outer membrane; cell soma; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; perikaryon; nucleus

Molecular Function: argininosuccinate lyase activity

Biological Process: amino acid metabolic process; response to drug; response to peptide hormone stimulus; ammonia assimilation cycle; arginine biosynthetic process; response to toxin; locomotory behavior; response to lipopolysaccharide; liver development; post-embryonic development; response to steroid hormone stimulus; response to zinc ion; midgut development; arginine biosynthetic process via ornithine; internal protein amino acid acetylation; kidney development; argininosuccinate metabolic process; response to nutrient; response to amine stimulus; aging; urea cycle