Rabbit anti-Human Hck Polyclonal Antibody | anti-Hck antibody

Hck, NT (Hck, Tyrosine-protein kinase HCK, B Cell/myeloid kinase, Hematopoietic cell kinase, Hemopoietic cell kinase, p56-HCK/p59-HCK) (PE)

Cat. Num. AAA6305789

• Gene Names: Hck; Bmk; AI849071
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Hck; Polyclonal Antibody; NT (Hck; Tyrosine-protein kinase HCK; B Cell/myeloid kinase; Hematopoietic cell kinase; Hemopoietic cell kinase; p56-HCK/p59-HCK) (PE); B-cell/myeloid kinase; p56-HCK/p59-HCK; anti-Hck antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 524

• Applicable Applications for anti-Hck antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Mouse Hck antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-101 amino acids from the N-terminal region of Mouse Hck.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Hck Antibody (N-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the Hck antibody detected the Hck protein (arrow).)

Related Product Information for anti-Hck antibody

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Product Categories/Family for anti-Hck antibody

Antibodies; Protein Kinases

References

Verollet, C., et al. J. Immunol. 184(12):7030-7039(2010). Ruschmann, J., et al. Exp. Hematol. 38(5):392-402(2010). Lowell, C.A., et al. J. Cell Biol. 133(4):895-910(1996). Lowell, C.A., et al. Blood 87(5):1780-1792(1996). Avraham, K.B., et al. Genomics 13(2):264-268(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 34734056
• NCBI GeneID: 15162
• NCBI Accession #: NP_034537.2; NP_001165588.1
• NCBI GenBank Nucleotide #: NP_034537.2
• UniProt Accession #: P08103
• NCBI Official Full Name: tyrosine-protein kinase HCK isoform p59Hck
• NCBI Official Synonym Full Names: hemopoietic cell kinase
• NCBI Official Symbol: Hck
• NCBI Official Synonym Symbols: Bmk; AI849071
• NCBI Protein Information: tyrosine-protein kinase HCK
• UniProt Protein Name: Tyrosine-protein kinase HCK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: Hck
• UniProt Synonym Gene Names: BMK

NCBI Description

The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may play a role in the innate immune response and the STAT5 signaling pathway. Alternative translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization. [provided by RefSeq, Feb 2010]

Uniprot Description

Non-receptor tyrosine-protein kinase found in hematopoietic cells that transmits signals from cell surface receptors and plays an important role in the regulation of innate immune responses, including neutrophil, monocyte, macrophage and mast cell functions, phagocytosis, cell survival and proliferation, cell adhesion and migration. Acts downstream of receptors that bind the Fc region of immunoglobulins, such as FCGR1A and FCGR2A, but also CSF3R, PLAUR, the receptors for IFNG, IL2, IL6 and IL8, and integrins, such as ITGB1 and ITGB2. During the phagocytic process, mediates mobilization of secretory lysosomes, degranulation, and activation of NADPH oxidase to bring about the respiratory burst. Plays a role in the release of inflammatory molecules. Promotes reorganization of the actin cytoskeleton and actin polymerization, formation of podosomes and cell protrusions. Inhibits TP73-mediated transcription activation and TP73-mediated apoptosis. Phosphorylates CBL in response to activation of immunoglobulin gamma Fc region receptors. Phosphorylates ADAM15, BCR, ELMO1, FCGR2A, GAB1, GAB2, RAPGEF1, STAT5B, TP73, VAV1 and WAS ().