Typical Testing Data/Standard Curve (for reference only)
Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit | AOC3 elisa kit
Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit
Gene Names
Aoc3; SSAO
Reactivity
Rat
Synonyms
Amine Oxidase; Copper Containing 3 (AOC3); Rat Amine Oxidase; Copper Containing 3 (AOC3) ELISA Kit; VAP1; HPAO; SSAO; Membrane primary amine oxidase; Vascular Adhesion Protein 1; Semicarbazide-Sensitive Amine Oxidase; Copper-Containing Amine Oxygen Oxidoreductase; AOC3 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of AOC3. No significant cross-reactivity or interference between AOC3 and analogues was observed.
Sequence Length
763
Samples
Serum, plasma, tissue homogenates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.117ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AOC3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AOC3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AOC3 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of AOC3 in rat serum, plasma, tissue homogenates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AOC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AOC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AOC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AOC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AOC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to AOC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AOC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AOC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,981 Da
NCBI Official Full Name
membrane primary amine oxidase
NCBI Official Synonym Full Names
amine oxidase, copper containing 3
NCBI Official Symbol
Aoc3
NCBI Official Synonym Symbols
SSAO
NCBI Protein Information
membrane primary amine oxidase
UniProt Protein Name
Membrane primary amine oxidase
Protein Family
UniProt Gene Name
Aoc3
UniProt Synonym Gene Names
SSAO; VAP-1
NCBI Description
localized to the adipocyte plasma membrane and to glucose transporter Glut4 containing vesicles [RGD, Feb 2006]
Uniprot Description
Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis ().
Research Articles on AOC3
Similar Products
Product Notes
The Rat AOC3 aoc3 (Catalog #AAA456955) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA456955 ELISA Kit recognizes Rat AOC3. It is sometimes possible for the material contained within the vial of "Amine Oxidase, Copper Containing 3 (AOC3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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