Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit | AOC3 elisa kit

Rat Amine Oxidase, Copper Containing 3 (AOC3) ELISA Kit

Cat. Num. AAA2097996

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Reactivity: Rat
• Synonyms: Amine Oxidase; Copper Containing 3 (AOC3); Rat Amine Oxidase; Copper Containing 3 (AOC3) ELISA Kit; VAP1; HPAO; SSAO; Membrane primary amine oxidase; Vascular Adhesion Protein 1; Semicarbazide-Sensitive Amine Oxidase; Copper-Containing Amine Oxygen Oxidoreductase; AOC3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Amine Oxidase, Copper Containing 3 (AOC3).; No significant cross-reactivity or interference between Amine Oxidase, Copper Containing 3 (AOC3) and analogues was observed.
• Sequence Length: 763

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.7pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amine Oxidase, Copper Containing 3 (AOC3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amine Oxidase, Copper Containing 3 (AOC3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AOC3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Amine Oxidase, Copper Containing 3 (AOC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Amine Oxidase, Copper Containing 3 (AOC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Amine Oxidase, Copper Containing 3 (AOC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Amine Oxidase, Copper Containing 3 (AOC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for AOC3 elisa kit

Enzyme & Kinase; Infection immunity; Endocrinology; Bone metabolism; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 29792247
• NCBI GeneID: 8639
• Molecular Weight: 24,533 Da
• NCBI Official Full Name: Amine oxidase, copper containing 3 (vascular adhesion protein 1)
• NCBI Official Synonym Full Names: amine oxidase, copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase
• UniProt Protein Name: Membrane primary amine oxidase
• Protein Family: Allene oxide cyclase
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1

NCBI Description

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - tyrosine; Cell adhesion; EC 1.4.3.21; Membrane protein, integral; Other Amino Acids Metabolism - beta-alanine; Oxidoreductase

Chromosomal Location of Human Ortholog: 17q21.31

Cellular Component: cell surface; cytoplasm; early endosome; endoplasmic reticulum; Golgi apparatus; integral component of membrane; microvillus; plasma membrane

Molecular Function: amine oxidase activity; calcium ion binding; copper ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; quinone binding

Biological Process: amine metabolic process; cell adhesion; inflammatory response; response to antibiotic; xenobiotic metabolic process