Human A3/ADORA3 (Adenosine A3 Receptor) ELISA Kit | A3/ADORA3 elisa kit

Human A3/ADORA3 (Adenosine A3 Receptor) ELISA Kit

Cat. Num. AAA7607033

• Reactivity: Human
• Synonyms: A3/ADORA3 (Adenosine A3 Receptor); Human A3/ADORA3 (Adenosine A3 Receptor) ELISA Kit; A3/ADORA3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of A3/ADORA3. No significant cross-reactivity or interference between A3/ADORA3 and analogues was observed.
• Sequence Length: 318

• Samples: serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A3/ADORA3 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A3/ADORA3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for A3/ADORA3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- A3/ADORA3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-A3/ADORA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the A3/ADORA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of A3/ADORA3 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 718550823
• NCBI GeneID: 704295
• NCBI Accession #: NP_001289711.1
• NCBI GenBank Nucleotide #: NM_001302782.1
• NCBI Official Full Name: adenosine A3 receptor
• NCBI Official Symbol: ADORA3
• NCBI Protein Information: adenosine A3 receptor

NCBI Description

This gene encodes a protein that belongs to the family of adenosine receptors, which are G-protein-coupled receptors involved in a variety of intracellular signaling pathways and physiological functions. Studies of orthologous proteins suggest that the receptor encoded by this gene mediates a sustained cardioprotective function during cardiac ischemia, it is involved in the inhibition of neutrophil degranulation in neutrophil-mediated tissue injury, it may have neuroprotective and neurodegenerative effects, and it may also mediate cell proliferation and cell death. This gene shares its 5' terminal exon with some transcripts from overlapping GeneID:104564310, which encodes a transmembrane and immunoglobulin domain-containing protein. [provided by RefSeq, Nov 2014]