Typical Testing Data/Standard Curve (for reference only)
(Human Ficolin-2 PicoKine ELISA Kit standard curve )
Human Ficolin-2 ELISA Kit | FCN2 elisa kit
Human Ficolin-2 PicoKine ELISA Kit
Gene Names
FCN2; P35; FCNL; EBP-37; ficolin-2
Reactivity
Human
Synonyms
Ficolin-2; Human Ficolin-2 PicoKine ELISA Kit; ficolin (collagen/fibrinogen domain containing lectin) 2; 37 kDa elastin-binding protein; Collagen/fibrinogen domain containing protein 2; Collagen/fibrinogen domain-containing protein 2; EBP 37; EBP-37; EBP37; FCN 2; Fcn2; FCN2_HUMAN; FCNL; Ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); Ficolin B; Ficolin beta; Ficolin-B; Ficolin-beta; Ficolin2; Hucolin; L ficolin; L-ficolin; OTTHUMP00000022518; P35; RP11 263F14.2; Serum lectin p35; FCN2 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human Ficolin-2
Sequence Length
313
Samples
Cell culture supernates, serum and plasma(heparin).
Assay Type
Sandwich
Detection Range
0.78ng/ml-50ng/ml
Sensitivity
< 20pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human Ficolin-2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human Ficolin-2 PicoKine ELISA Kit standard curve )
Related Product Information for FCN2 elisa kit
Principle of the Assay: The nL;man Ficolin-2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay :echno;ogy. A monoclonal antibody from mouse specific for Ficolin-2 has been precoated onto 96-well plates. Standards(NSO. L26-A313) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Ficolin-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Ficolin-2 amount of sample captured in plate.
Background/Introduction: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide. a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.
Background/Introduction: Ficolin-2, which was initially identified as L-ficolin, is a protein that in humans is encoded by the FCN2 gene. The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide. a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. It is mapped to 9q34.3. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Serum concentration of ficolin-2 varies considerably in healthy individuals. FCN2 promoter polymorphisms were associated with marked changes in theficolin-2 serum concentration.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
30,228 Da
NCBI Official Full Name
Ficolin-2
NCBI Official Synonym Full Names
ficolin (collagen/fibrinogen domain containing lectin) 2
NCBI Official Symbol
FCN2
NCBI Official Synonym Symbols
P35; FCNL; EBP-37; ficolin-2
NCBI Protein Information
ficolin-2; L-ficolin; ficolin B; ficolin-B; ficolin-beta; serum lectin p35; 37 kDa elastin-binding protein; collagen/fibrinogen domain-containing protein 2; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)
UniProt Protein Name
Ficolin-2
Protein Family
UniProt Gene Name
FCN2
UniProt Synonym Gene Names
FCNL
UniProt Entry Name
FCN2_HUMAN
NCBI Description
The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
FCN2: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. Belongs to the ficolin lectin family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 9q34
Research Articles on FCN2
Similar Products
Product Notes
The Human FCN2 fcn2 (Catalog #AAA177113) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177113 ELISA Kit recognizes Human FCN2. It is sometimes possible for the material contained within the vial of "Ficolin-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
