Rat Acti-vated protein C (APC) ELISA Kit | APC elisa kit

Rat Acti-vated protein C (APC) ELISA Kit

Cat. Num. AAA268506

• Gene Names: APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• Reactivity: Rat
• Synonyms: Acti-vated protein C (APC); Rat Acti-vated protein C (APC) ELISA Kit; APC elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 2843

• Samples: Rat serum, plasma or cell culture supernatant
• Detection Range: 10 ng/ml-0.156ng/ml
• Sensitivity: 0.05 ng/mL
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APC elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat APC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029.2
• NCBI GenBank Nucleotide #: NM_000038.5
• Molecular Weight: 300,439 Da
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: adenomatous polyposis coli
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: Adenomatous polyposis coli
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; beta-catenin destruction complex; cytosol; kinetochore; nucleoplasm; cell-cell adherens junction; cytoplasmic microtubule; lamellipodium; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; apoptosis; positive regulation of apoptosis; somatic stem cell maintenance; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; negative regulation of cell proliferation; regulation of osteoclast differentiation; hair follicle development; cytokinesis after mitosis; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell cycle arrest; cell adhesion; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of attachment of spindle microtubules to kinetochore; cell migration; thymus development; regulation of osteoblast differentiation; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma