Typical Testing Data/Standard Curve (for reference only)
Rat A Disintegrin And Metalloprotease 15, ADAM15 ELISA Kit | ADAM15 elisa kit
Rat A Disintegrin And Metalloprotease 15, ADAM15 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AD antibody and an AD-HRP conjugate. The assay sample and buffer are incubated together with AD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AD concentration since AD from samples and AD-HRP conjugate compete for the anti-AD antibody binding site. Since the number of sites is limited, as more sites are occupied by AD from the sample, fewer sites are left to bind AD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AD concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Uniprot Description
ADAM15: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain. 11 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Cell adhesion; EC 3.4.24.-; Membrane protein, integral; Protease
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: cell surface; cell projection; adherens junction; integral to membrane; acrosome; plasma membrane
Molecular Function: integrin binding; protein binding; zinc ion binding; metallopeptidase activity; metalloendopeptidase activity; SH3 domain binding
Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; apoptosis; cell-matrix adhesion; proteolysis; negative regulation of cell-matrix adhesion; extracellular matrix disassembly; collagen catabolic process; tissue regeneration; innate immune response; angiogenesis; negative regulation of cell growth; negative regulation of cell migration
Research Articles on ADAM15
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Product Notes
The Rat ADAM15 adam15 (Catalog #AAA7212927) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212927 ELISA Kit recognizes Rat ADAM15. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 15, ADAM15, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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