Western Blot (WB)
(TORC2 antibody (pAb) tested by Immunoprecipitation. 10 ul of TORC2 antibody was used to immunoprecipitate TORC2 from 400 ug of HEK293 whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the TORC2 antibody at a dilution of 1:2,000.)
Rabbit anti-Human, Mouse TORC2 Polyclonal Antibody | anti-TORC2 antibody
TORC2 antibody (pAb)
IP: 5-10ul per IP
WB: 1:500-1:2,000 dilution
Western Blot (WB)
(TORC2 antibody (pAb) tested by Immunoprecipitation. 10 ul of TORC2 antibody was used to immunoprecipitate TORC2 from 400 ug of HEK293 whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the TORC2 antibody at a dilution of 1:2,000.)
Western Blot (WB)
(TORC2 antibody (pAb) tested by Immunoprecipitation. 10 ul of TORC2 antibody was used to immunoprecipitate TORC2 from 400 ug of HEK293 whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the TORC2 antibody at a dilution of 1:2,000.)
Western Blot (WB)
(TORC2 antibody (pAb) tested by Western blot. Detection of TORC2 by Western blot analysis. Whole cell extract (20 ug) of NIH/3T3 (lane 1) and HEK293 (lane 2) blotted with TORC2 antibody at a dilution of 1:2,000.)
Western Blot (WB)
(TORC2 antibody (pAb) tested by Western blot. Detection of TORC2 by Western blot analysis. Whole cell extract (20 ug) of NIH/3T3 (lane 1) and HEK293 (lane 2) blotted with TORC2 antibody at a dilution of 1:2,000.)
Background: TORC2 (Transducer of regulated cAMP response element-binding protein 2)(CRTC2 or CREB-regulated transcription coactivator 2) is a coactivator of transcription through the CREB1 signaling pathway, independent of phosphorylation at serine 133. Involved in metabolic signaling pathways, it is responsive to glucagon signaling and regulates gluconeogenesis. It is a potent activator of mitochondrial biogenesis in muscle cells. TORC2 is inactivated by phosphorylation at serine 171 (in response to both insulin signaling and the activation of AMPK), which facilitates recognition by 14-3-3 protein and subsequent translocation to the cytoplasm.
NCBI and Uniprot Product Information
Observed: 70 kDa
NCBI Description
This gene encodes a member of the transducers of regulated cAMP response element-binding protein activity family of transcription coactivators. These proteins promote the transcription of genes targeted by the cAMP response element-binding protein, and therefore play an important role in many cellular processes. Under basal conditions the encoded protein is phosphorylated by AMP-activated protein kinase or the salt-inducible kinases and is sequestered in the cytoplasm. Upon activation by elevated cAMP or calcium, the encoded protein translocates to the nucleus and increases target gene expression. Single nucleotide polymorphisms in this gene may increase the risk of type 2 diabetes. A pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2010]
Uniprot Description
TORC2: the transducer of regulated CREB protein 2 (TORC2) is a cAMP responsive coactivator that, in concert with LKB1 and AMPK, controls glucose homeostasis in the liver. Under fasting conditions, cytoplasmic TORC2 is transported to the nucleus where it binds to CREB and stimulates gluconeogenisis. Re-feeding and the ensuing insulin response inhibits gluconeogenic gene expression by promoting the phosphorylation, cytoplasmic export and degradation of TORC2. TORC2 is phosphorylated by the kinase QIK which in turn is activated by phosphorylation at Ser358 by insulin-activated Akt2. Phosphorylated TORC2 is subsequently ubiquitinylated at Lys 628 and degraded by the 26S proteasome. TORC2 protein levels and activity are increased in diabetes owing to a block in TORC2 phosphorylation, implicating this pathway in the pathogenesis of diabetes. The CREB/TORC2 regulatory axis controls the normal pattern of germinal center (GC) B cell gene activation/repression that promotes B cell development and circumvents GC lymphomagenesis.
Protein type: Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 1q21.3
Cellular Component: nucleoplasm; cytoplasm
Molecular Function: protein binding; cAMP response element binding protein binding
Biological Process: viral reproduction; transcription, DNA-dependent; activation of CREB transcription factor; positive regulation of transcription from RNA polymerase II promoter; glucose homeostasis; gluconeogenesis; protein homotetramerization
Research Articles on TORC2
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Product Notes
The TORC2 crtc2 (Catalog #AAA388574) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TORC2 antibody (pAb) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TORC2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 5-10ul per IP WB: 1:500-1:2,000 dilution. Researchers should empirically determine the suitability of the TORC2 crtc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TORC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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