Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit | HTR2C elisa kit

Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit

Cat. Num. AAA2705133

• Gene Names: HTR2C; HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C
• Reactivity: Rat
• Synonyms: 5-Hydroxytryptamine Receptor 2C (HTR2C); Rat 5-Hydroxytryptamine Receptor 2C (HTR2C) ELISA Kit; 5-HT2C; HTR1C; Serotonin Receptor 2C; 5-hydroxytryptamine receptor 1C; HTR2C elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of 5-Hydroxytryptamine Receptor 2C (HTR2C).; No significant cross-reactivity or interference between 5-Hydroxytryptamine Receptor 2C (HTR2C) and analogues was observed.
• Sequence Length: 248

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 2C (HTR2C) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level 5-Hydroxytryptamine Receptor 2C (HTR2C) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HTR2C elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-Hydroxytryptamine Receptor 2C (HTR2C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 5-Hydroxytryptamine Receptor 2C (HTR2C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5-Hydroxytryptamine Receptor 2C (HTR2C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5-Hydroxytryptamine Receptor 2C (HTR2C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4504541
• NCBI GeneID: 3358
• NCBI Accession #: NP_000859.1
• NCBI GenBank Nucleotide #: NM_000868.3
• UniProt Accession #: P28335; Q5VUF8; Q9NP28; B1AMW4
• Molecular Weight: 28,055 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 2C isoform a
• NCBI Official Synonym Full Names: 5-hydroxytryptamine receptor 2C
• NCBI Official Symbol: HTR2C
• NCBI Official Synonym Symbols: HTR1C; 5-HT1C; 5-HT2C; 5HTR2C; 5-HTR2C
• NCBI Protein Information: 5-hydroxytryptamine receptor 2C
• UniProt Protein Name: 5-hydroxytryptamine receptor 2C
• Protein Family: 5-hydroxytryptamine receptor
• UniProt Gene Name: HTR2C
• UniProt Synonym Gene Names: HTR1C; 5-HT-2C; 5-HT2C; 5-HTR2C; 5-HT-1C; 5-HT1C

NCBI Description

This gene encodes a seven-transmembrane G-protein-coupled receptor. The encoded protein responds to signaling through the neurotransmitter serotonin. The mRNA of this gene is subject to multiple RNA editing events, where adenosine residues encoded by the genome are converted to inosines. RNA editing is predicted to alter the structure of the second intracellular loop, thereby generating alternate protein forms with decreased ability to interact with G proteins. Abnormalities in RNA editing of this gene have been detected in victims of suicide that suffer from depression. In addition, naturally-occuring variation in the promoter and 5' non-coding and coding regions of this gene may show statistically-significant association with mental illness and behavioral disorders. Alternative splicing results in multiple different transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including ergot alkaloid derivatives, 1-2,5,-dimethoxy-4-iodophenyl-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and down-stream signaling cascades and promotes the release of Ca2+ ions from intracellular stores. Regulates neuronal activity via the activation of short transient receptor potential calcium channels in the brain, and thereby modulates the activation of pro-opiomelacortin neurons and the release of CRH that then regulates the release of corticosterone. Plays a role in the regulation of appetite and eating behavior, responses to anxiogenic stimuli and stress. Plays a role in insulin sensitivity and glucose homeostasis.