Rat 5-hydroxy tryptamine (5-ht) ELISA Kit | 5-ht elisa kit

Rat 5-hydroxy tryptamine (5-ht) ELISA Kit

Cat. Num. AAA266539

• Gene Names: HTR1A; G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
• Reactivity: Rat
• Synonyms: 5-hydroxy tryptamine (5-ht); Rat 5-hydroxy tryptamine (5-ht) ELISA Kit; 5-ht elisa kit

• Reactivity: Rat
• Sequence Length: 422

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 100 ng/mL-1.56 ng/mL
• Sensitivity: Up to 0.5 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for 5-ht elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat 5-ht monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 53748104
• NCBI GeneID: 3350
• UniProt Accession #: Q5ZGX3; P08908
• Molecular Weight: 46,107 Da
• NCBI Official Full Name: 5-hydroxytryptamine receptor 1A
• NCBI Official Synonym Full Names: 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled
• NCBI Official Symbol: HTR1A
• NCBI Official Synonym Symbols: G-21; 5HT1a; PFMCD; 5-HT1A; 5-HT-1A; ADRBRL1; ADRB2RL1
• NCBI Protein Information: 5-hydroxytryptamine receptor 1A; 5-HT1a receptor; guanine nucleotide-binding regulatory protein-coupled receptor
• UniProt Protein Name: 5-hydroxytryptamine (Serotonin) receptor 1A
• UniProt Gene Name: 5HT1A
• UniProt Synonym Gene Names: HTR1A; hCG_16836
• UniProt Entry Name: Q5ZGX3_HUMAN

NCBI Description

This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]

Uniprot Description

5-HT(1A): This is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Belongs to the G-protein coupled receptor 1 family. 5- hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.

Protein type: Receptor, GPCR; Membrane protein, integral; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q11.2-q13

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding; serotonin receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell proliferation; vasoconstriction; behavioral fear response; serotonin receptor, adenylate cyclase inhibiting pathway; regulation of dopamine metabolic process; positive regulation of cell proliferation; regulation of serotonin secretion; regulation of hormone secretion; serotonin receptor signaling pathway; serotonin metabolic process; regulation of behavior

Disease: Periodic Fever, Menstrual Cycle-dependent